Nowadays, signal enhancement is imperative to increase sensitivity of advanced ECL devices for expediting their promising applications in clinic. In this work, photodynamic-assisted electrochemiluminescence (PDECL) device was constructed for precision diagnosis of Parkinson, where an advanced emitter was prepared by electrostatically linking 2,6-dimethyl-8-(3-carboxyphenyl)4,4′-difluoroboradiazene (BET) with 1-butyl-3-methylimidazole tetrafluoroborate ([BMIm][BF₄]). Specifically, protoporphyrin IX (PPIX) can trigger the photodynamic reaction under light irradiation with a wavelength of 450 nm to generate lots of singlet oxygen (¹O₂), showing a 2.43-fold magnification in the ECL responses. Then, the aptamer (Apt) was assembled on the functional BET-[BMIm] for constructing a “signal off” ECL biosensor. Later on, the PPIX was embedded into the G-quadruplex (G4) of the Apt to magnify the ECL signals for bioanalysis of α-synuclein (α-syn) under light excitation. In the optimized surroundings, the resulting PDECL sensor has a broad linear range of 100.0 aM ∼ 10.0 fM and a low limit of detection (LOD) of 63 aM, coupled by differentiating Parkinson patients from normal individuals according to the receiver operating characteristic (ROC) curve analysis of actual blood samples. Such research holds great promise for synthesis of other advanced luminophores, combined with achieving an early clinical diagnosis.

中文翻译：

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光动力辅助电化学发光增强先进 BODIPY 用于帕金森病的精确诊断


如今，信号增强对于提高先进 ECL 设备的灵敏度至关重要，以加快其在临床上的应用。在这项工作中，构建了用于精确诊断帕金森病的光动力辅助电化学发光（PDECL）装置，其中通过静电连接2,6-二甲基-8-(3-羧基苯基)4,4'-二氟硼二氮烯（BET）制备了先进的发射器）与 1-丁基-3-甲基咪唑四氟硼酸盐（[BMIm][BF ₄ ]）。具体来说，原卟啉IX（PPIX）在450 nm波长的光照射下可以引发光动力反应，产生大量的单线态氧（ ¹ O ₂ ），表现出2.43倍的光动力反应。 ECL 响应的放大倍数。然后，将适体（Apt）组装在功能性BET-[BMIm]上，构建“信号关闭”ECL生物传感器。随后，将 PPIX 嵌入 Apt 的 G-四链体 (G4) 中，以放大 ECL 信号，以便在光激发下对 α-突触核蛋白 (α-syn) 进行生物分析。在优化的环境中，所得的 PDECL 传感器具有 100.0 aM ∼ 10.0 fM 的宽线性范围和 63 aM 的检测下限 (LOD)，同时根据接收器操作特征 (ROC) 将帕金森患者与正常个体区分开来实际血液样本的曲线分析。此类研究为合成其他先进发光体以及实现早期临床诊断带来了巨大希望。