In this study, we reported a selective impedimetric biosensor for the detection of A29 which is the target protein of the monkeypox virus (MPXV). The working principle of the biosensor relies on the interaction mechanism between A29, which is an internal membrane protein of MPXV, and the heparan sulfate receptor. For this purpose, after immobilizing heparan sulfate onto the gold screen-printed electrode surface, its interaction with A29 protein was monitored using electrochemical impedance spectroscopy. After the optimization of experimental parameters, the analytical characteristics of the developed MPVX immunosensor were examined. The developed immunosensor exhibited a linear detection range between 2.0 and 50 ng mL^–1, with a detection limit of 2.08 ng mL^–1 and a quantification limit of 6.28 ng mL^–1. Furthermore, a relative standard deviation value of 2.82% was determined for 25 ng mL^–1. Apart from that, sample application studies were also performed with the standard addition of A29 protein to 1:10 diluted real serum samples that were taken from healthy individuals, and very good recovery values were obtained.

中文翻译：

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用于人猴痘病毒检测的电化学免疫分析平台


在这项研究中，我们报道了一种选择性阻抗生物传感器，用于检测猴痘病毒（MPXV）的靶蛋白A29。该生物传感器的工作原理依赖于MPXV内膜蛋白A29与硫酸乙酰肝素受体之间的相互作用机制。为此，将硫酸乙酰肝素固定到金丝网印刷电极表面后，使用电化学阻抗谱监测其与 A29 蛋白的相互作用。优化实验参数后，对所开发的 MPVX 免疫传感器的分析特性进行了检验。所开发的免疫传感器的线性检测范围为2.0至50 ng mL ^–1 ，检测限为2.08 ng mL ^–1 ，定量限为6.28 ng mL ^–1 的相对标准偏差值为 2.82%。除此之外，还进行了样品应用研究，将 A29 蛋白标准添加到取自健康个体的 1:10 稀释真实血清样品中，并获得了非常好的回收值。