A novel millifluidic process introduces age-based fractionation of S. pastorianus var. carlsbergensis yeast culture through magnetophoresis. Saccharomyces yeast is a model organism for aging research used in various industries. Traditional age-based cell separation methods were labor-intensive, but techniques like magnetic labeling have eased the process by being non-invasive and scalable. Our approach introduces an age-specific fractionation using a 3D-printed millfluidic chip in a two-step process, ensuring efficient cell deflection in the magnetic field and counteracting magnetic induced convection. Among various channel designs, the pinch-shaped channel proved most effective for age differentiation based on magnetically labeled bud scar numbers. Metabolomic analyses revealed changes in certain amino acids and increased NAD⁺ levels, suggesting metabolic shifts in aging cells. Gene expression studies further underlined these age-related metabolic changes. This innovative platform offers a high-throughput, non-invasive method for age-specific yeast cell fractionation, with potential applications in industries ranging from food and beverages to pharmaceuticals.

中文翻译：

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用于年龄特异性分级的微流控磁泳芯片：评估年龄对酵母代谢组学和基因表达的影响

一种新颖的微流体过程引入了基于年龄的巴斯德酵母变种的分级分离。通过磁泳进行嘉士伯酵母培养。酵母菌是一种用于各个行业的衰老研究的模式生物。传统的基于年龄的细胞分离方法是劳动密集型的，但磁性标记等技术通过非侵入性和可扩展性简化了该过程。我们的方法在两步过程中使用 3D 打印的研磨流体芯片引入了年龄特异性分级，确保磁场中细胞的有效偏转并抵消磁感应对流。在各种通道设计中，捏形通道被证明对于基于磁性标记的芽痕数量的年龄区分最有效。代谢组学分析揭示了某些氨基酸的变化和 NAD ⁺水平的增加，表明衰老细胞的代谢发生变化。基因表达研究进一步强调了这些与年龄相关的代谢变化。这一创新平台为特定年龄的酵母细胞分离提供了一种高通量、非侵入性的方法，在食品、饮料和制药等行业具有潜在的应用前景。