This study investigated the influence of yeast extract addition, carbon source, and photoperiod on the growth dynamics of FACHB-5. Employing response surface methodology, the culture strategy was optimized, resulting in the following optimal conditions: yeast extract addition at 0.75 g L, glucose concentration of 0.83 g L, and a photoperiod set at Light: Dark = 18 h: 6 h. Under these conditions, the biomass reached 1.76 g L with a protein content of 750.00 g L, containing 40 % of essential amino acids, representing a 1.52-fold increase. Proteomic analysis revealed that the targeted cultivation strategy up-regulated genes involved in microalgal protein synthesis. The combined effect of yeast extract and glucose enhanced both the glutamine synthetase-glutamate synthetase mechanism and the free amino acid content.

中文翻译：

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研究提高蛋白核小球藻 FACHB-5 蛋白质含量的培养策略


本研究调查了酵母提取物添加、碳源和光周期对 FACHB-5 生长动态的影响。采用响应面法优化培养策略，得到以下最佳条件：酵母提取物添加量为0.75 g·L，葡萄糖浓度为0.83 g·L，光周期设置为光照：黑暗= 18 h：6 h。在此条件下，生物量达到1.76 g·L，蛋白质含量为750.00 g·L，含有40%的必需氨基酸，增加了1.52倍。蛋白质组学分析表明，靶向培养策略上调了参与微藻蛋白质合成的基因。酵母提取物和葡萄糖的联合作用增强了谷氨酰胺合成酶-谷氨酸合成酶机制和游离氨基酸含量。