The surface engineering of biomaterials is crucial for their successful (bio)integration by the body, i.e. the colonization by the tissue-specific cell, and the prevention of fibrosis and/or bacterial colonization. Performed at room temperature in an aqueous medium, the layer-by-layer (LbL) coating method is based on the alternating deposition of macromolecules. Versatile and simple, this method allows the functionalization of surfaces with proteins, which play a crucial role in several biological mechanisms. Possessing intrinsic properties (cell adhesion, antibacterial, degradable, etc.), protein-based LbL films represent a powerful tool to control bacterial and mammalian cell fate. In this article, after a general introduction to the LbL technique, we will focus on protein-based LbL films addressing different biomedical issues/domains, such as bacterial infection, blood contacting surfaces, mammalian cell adhesion, drug and gene delivery, and bone and neural tissue engineering. We do not consider biosensing applications or electrochemical aspects using specific proteins such as enzymes.

中文翻译：

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用于生物医学应用的基于蛋白质的逐层薄膜


生物材料的表面工程对于它们在体内的成功（生物）整合（即组织特异性细胞的定植以及防止纤维化和/或细菌定植）至关重要。逐层 (LbL) 涂层方法在室温下在水性介质中进行，基于大分子的交替沉积。这种方法用途广泛且简单，可以用蛋白质对表面进行功能化，这在多种生物机制中发挥着至关重要的作用。基于蛋白质的 LbL 薄膜具有内在特性（细胞粘附、抗菌、可降解等），是控制细菌和哺乳动物细胞命运的强大工具。在本文中，在对 LbL 技术进行一般介绍后，我们将重点关注基于蛋白质的 LbL 薄膜，解决不同的生物医学问题/领域，例如细菌感染、血液接触表面、哺乳动物细胞粘附、药物和基因传递以及骨和神经组织工程。我们不考虑使用特定蛋白质（例如酶）的生物传感应用或电化学方面。