Plants provide a wide array of compounds that can be explored for potential anticancer properties. Siphonochilone, a furanoterpene that represents one of the main components of the African plant Siphonochilus aethiopicus, shows numerous health benefits. However, to date, its antiproliferative properties have not been tested. The aim of this study was to analyze the cytotoxic effects of siphonochilone on a panel of cancer cell lines and its underlying mechanism of action. Our results demonstrated that siphonochilone exhibited significant cytotoxic effects on pancreatic, breast, lung, colon, and liver cancer cell lines showing a IC50 ranging from 22 to 124 μM at 72 h of treatment and highlighting its cytotoxic effect against MCF7 and PANC1 breast and pancreas cancer cell lines (22.03 and 39.03 μM, respectively). Cell death in these tumor lines was mediated by apoptosis by the mitochondrial pathway, as evidenced by siphonochilone‐induced depolarization of the mitochondrial membrane potential. In addition, siphonochilone treatment involves the generation of reactive oxygen species that may contribute to apoptosis induction. In this work, we described for the first time the cytotoxic properties of siphonochilone and provided data about the molecular processes of cell death. Although future studies will be necessary, our results support the interest in this molecule in relation to their clinical application in cancer, and especially in breast and pancreatic cancer.

中文翻译：

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非洲生姜 siphonochilone 的细胞毒作用评价：体外分析

植物提供了多种化合物，可探索其潜在的抗癌特性。 Siphonochilone，一种呋喃萜，是非洲植物的主要成分之一埃塞俄比亚管唇线虫，显示出许多健康益处。然而，迄今为止，其抗增殖特性尚未经过测试。本研究的目的是分析 siphonochilone 对一组癌细胞系的细胞毒性作用及其潜在作用机制。我们的结果表明 siphonochilone 对胰腺癌、乳腺癌、肺癌、结肠癌和肝癌细胞系表现出显着的细胞毒作用，显示出 IC50治疗 72 小时时浓度范围为 22 至 124 μM，突出显示其对 MCF7 和 PANC1 乳腺癌和胰腺癌细胞系（分别为 22.03 和 39.03 μM）的细胞毒性作用。这些肿瘤系中的细胞死亡是由线粒体途径的细胞凋亡介导的，虹吸虫酮诱导的线粒体膜电位去极化证明了这一点。此外，虹吸虫酮治疗涉及活性氧的产生，这可能有助于细胞凋亡的诱导。在这项工作中，我们首次描述了 siphonochilone 的细胞毒性特性，并提供了有关细胞死亡分子过程的数据。尽管未来的研究是必要的，但我们的结果支持了人们对该分子在癌症（特别是乳腺癌和胰腺癌）中的临床应用的兴趣。