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Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform
Nature Communications ( IF 16.6 ) Pub Date : 2024-05-15 , DOI: 10.1038/s41467-024-48132-4
Alexia Stollmann , Jose Garcia-Guirado , Jae-Sang Hong , Pascal Rüedi , Hyungsoon Im , Hakho Lee , Jaime Ortega Arroyo , Romain Quidant

Label-free detection of multiple analytes in a high-throughput fashion has been one of the long-sought goals in biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics tools that can process small volumes of sample with high throughput, and with surface chemistry that grants analyte specificity, poses a critical challenge to date. Here, we introduce an optofluidic platform that brings together state-of-the-art digital holography with PDMS microfluidics by using supported lipid bilayers as a surface chemistry building block to integrate both technologies. Specifically, this platform fingerprints heterogeneous biological nanoparticle populations via a multiplexed label-free immunoaffinity assay with single particle sensitivity. First, we characterise the robustness and performance of the platform, and then apply it to profile four distinct ovarian cell-derived extracellular vesicle populations over a panel of surface protein biomarkers, thus developing a unique biomarker fingerprint for each cell line. We foresee that our approach will find many applications where routine and multiplexed characterisation of biological nanoparticles are required.



中文翻译:

使用无标记光流控平台对生物纳米颗粒进行分子指纹分析

以高通量方式对多种分析物进行无标记检测一直是生物传感应用中长期追求的目标之一。然而,对于全光学方法来说,将最先进的无标记技术与能够以高通量处理少量样品的微流体工具以及赋予分析物特异性的表面化学相结合,构成了迄今为止的严峻挑战。在这里,我们介绍了一个光流控平台,通过使用支持的脂质双层作为表面化学构建块来集成这两种技术,将最先进的数字全息术与 PDMS 微流控结合在一起。具体来说,该平台通过具有单颗粒灵敏度的多重无标记免疫亲和测定来对异质生物纳米颗粒群体进行指纹识别。首先,我们表征了该平台的稳健性和性能,然后将其应用于一组表面蛋白生物标志物上的四种不同的卵巢细胞衍生的细胞外囊泡群体的分析,从而为每个细胞系开发了独特的生物标志物指纹。我们预见,我们的方法将找到许多需要对生物纳米颗粒进行常规和多重表征的应用。

更新日期:2024-05-15
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