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A perfusion host‐microbe bioreactor (HMB) system that captures dynamic interactions of secreted metabolites between epithelial cells cocultured with a human gut anaerobe Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-08 Jingyun Yang, Jason Cassaday, Thomas P. Wyche, Brian Squadroni, William Newhard, Huong Trinh, Damien Cabral, Erik Hett, Theodore R. Sana, Kyongbum Lee, Stephen Kasper
The human microbiota impacts a variety of diseases and responses to therapeutics. Due to a lack of robust in vitro models, detailed mechanistic explanations of host‐microbiota interactions cannot often be recapitulated. We describe the design and development of a novel, versatile and modular in vitro system that enables indirect coculture of human epithelial cells with anaerobic bacteria for the characterization
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Vaccine process technology—A decade of progress Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-07 Barry Buckland, Gautam Sanyal, Todd Ranheim, David Pollard, Jim A. Searles, Sue Behrens, Stefanie Pluschkell, Jessica Josefsberg, Christopher J. Roberts
In the past decade, new approaches to the discovery and development of vaccines have transformed the field. Advances during the COVID‐19 pandemic allowed the production of billions of vaccine doses per year using novel platforms such as messenger RNA and viral vectors. Improvements in the analytical toolbox, equipment, and bioprocess technology have made it possible to achieve both unprecedented speed
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Higher efficiency of vanadate iron in heterogeneous Fenton‐like systems to pretreat sugarcane bagasse and its enzymatic saccharification Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-07 Ju Liang, Huiying Zeng, Yuting Zhang, Wenbing Zhou, Naidong Xiao
Pretreatment is crucial for effective enzymatic saccharification of lignocellulose such as sugarcane bagasse (SCB). In the present study, SCB was pretreated with five kinds of heterogeneous Fenton‐like systems (HFSs), respectively, in which α‐FeOOH, α‐Fe2O3, Fe3O4, and FeS2 worked as four traditional heterogeneous Fenton‐like catalysts (HFCs), while FeVO4 worked as a novel HFC. The enzymatic reducing
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Computational fluid dynamics based digital twins of fixed bed bioreactors validate scaling principles for recombinant adeno‐associated virus gene therapy manufacturing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-06 Michael Hill, Colten White, Shaoying Wang, John Thomas, Brian DeVincentis, Nripen Singh
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Evaluation of various membranes at different fluxes to enable large‐volume single‐use perfusion bioreactors Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-04 Hassan Raza, Tiffany Tang, Baizhen Gao, Chelsea Phuangthong, Chienhung Billy Chen, Nuno D. S. Pinto
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Reinventing shake flask fermentation: The breathable flask Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-03 Vikash Kumar, Michael Tolosa, Xudong Ge, Govind Rao
Cultivating cells in shake flasks is a routine operation that is largely unchanged since its inception. A glass or plastic Erlenmeyer vessel with the primary gas exchange taking place across various porous plugs is used with media volumes typically ranging from 100 mL to 2 L. Oxygen limitation and carbon dioxide accumulation in the vessel is a major concern for studies involving shake flask cultures
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Mechanistic modeling of in vitro transcription incorporating effects of magnesium pyrophosphate crystallization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-05-02 Nathan Merica Stover, Krystian Ganko, Richard D. Braatz
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Rapid spectrophotometric detection for optimized production of landomycins and characterization of their therapeutic potential Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-30 Todd C. Chappell, Kathleen G. Maiello, Allison J. Tierney, Karin Yanagi, Jessica A. Lee, Kyongbum Lee, Charles R. Mace, Clay S. Bennett, Nikhil U. Nair
Microbial‐derived natural products remain a major source of structurally diverse bioactive compounds and chemical scaffolds that have the potential as new therapeutics to target drug‐resistant pathogens and cancers. In particular, genome mining has revealed the vast number of cryptic or low‐yield biosynthetic gene clusters in the genus Streptomyces. However, low natural product yields—improvements
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Hotspot Wizard‐informed engineering of a hyperthermophilic β‐glucosidase for enhanced enzyme activity at low temperatures Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-29 Mehmet Emre Erkanli, Khalid El‐Halabi, Ted Keunsil Kang, Jin Ryoun Kim
Hyperthermophilic enzymes serve as an important source of industrial enzymes due to their high thermostability. Unfortunately, most hyperthermophilic enzymes suffer from reduced activity at low temperatures (e.g., ambient temperature), limiting their applicability. In addition, evolving hyperthermophilic enzymes to increase low temperature activity without compromising other desired properties is generally
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Listening to life: Sonification for enhancing discovery in biological research Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-28 Rhea Braun, Maxwell Tfirn, Roseanne M. Ford
Sonification, or the practice of generating sound from data, is a promising alternative or complement to data visualization for exploring research questions in the life sciences. Expressing or communicating data in the form of sound rather than graphs, tables, or renderings can provide a secondary information source for multitasking or remote monitoring purposes or make data accessible when visualizations
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Bioprocess in‐line monitoring and control using Raman spectroscopy and Indirect Hard Modeling (IHM) Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-28 David Heinrich Müller, Marieke Börger, Julia Thien, Hans‐Jürgen Koß
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Structural and molecular insights into a bifunctional glycoside hydrolase 30 xylanase specific to glucuronoxylan Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-28 Christina Pentari, Christos Kosinas, Efstratios Nikolaivits, Maria Dimarogona, Evangelos Topakas
Glycoside hydrolase (GH) 30 family xylanases are enzymes of biotechnological interest due to their capacity to degrade recalcitrant hemicelluloses, such as glucuronoxylan (GX). This study focuses on a subfamily 7 GH30, TtXyn30A from Thermothelomyces thermophilus, which acts on GX in an “endo” and “exo” mode, releasing methyl‐glucuronic acid branched xylooligosaccharides (XOs) and xylobiose, respectively
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Correction to “Metabolic engineering of betacyanin in vegetables for anti‐inflammatory therapy” Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-28
Saito, S., Nishihara, M., Kohakura, M., Kimura, K., Yashiro, T., Takasawa, S., & Arimura, G.-i. (2023). Metabolic engineering of betacyanin in vegetables for anti-inflammatory therapy. Biotechnology and Bioengineering, 120, 1357–1365. https://doi.org/10.1002/bit.28335 In the originally published article, in Section 2.8, second paragraph, “4,4-dimethyl-4-silapentane-1-sulfonic acid” should read “dextran
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Systems engineering Escherichia coli for efficient production p‐coumaric acid from glucose Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-26 Chong Qiu, Xiaoge Wang, Jiaojiao Zuo, Runyang Li, Cong Gao, Xiulai Chen, Jia Liu, Wanqing Wei, Jing Wu, Guipeng Hu, Wei Song, Nan Xu, Liming Liu
P‐coumaric acid (p‐CA), a pant metabolite with antioxidant and anti‐inflammatory activity, is extensively utilized in biomedicine, food, and cosmetics industry. In this study, a synthetic pathway (PAL) for p‐CA was designed, integrating three enzymes (AtPAL2, AtC4H, AtATR2) into a higher l‐phenylalanine‐producing strain Escherichia coli PHE05. However, the lower soluble expression and activity of AtC4H
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Synthesized arsenic nanoparticles and their high potential in biomedical applications: A review Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-26 Seyed Soheil Hosseininasab, Mahin Naderifar, Majid Reza Akbarizadeh, Nooshin Hashemi, Mehdi Ghaderi, Hamid Pajavand, Naghmeh Satarzadeh, Amin Sadeghi Dousari
Arsenic with the scientific name AS is an element that exists everywhere. It is the fourth among the abundant elements in water, the twelfth in the human body, and the twentieth in the earth's crust. This element exists in sulfide, carbonate, and elemental forms. Different names of arsenic are known as white arsenic (As2O3), yellow arsenic (As2S3), and red arsenic (As4S4). Nowadays, due to its unique
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Covalent organic frameworks (COFs) as carrier for improved drug delivery and biosensing applications Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-26 Rida Younas, Farhat Jubeen, Nargis Bano, Silvana Andreescu, Hongxia Zhang, Akhtar Hayat
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Super7 passaging method to improve Chinese hamster ovary cell fed‐batch performance Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-25 Matthew J. Moran, Jin Chen, James M. Piret, R. Robert Balcarcel
Chinese hamster ovary (CHO) cells are widely used to manufacture biopharmaceuticals, most of all monoclonal antibodies (mAbs). Some CHO cell lines exhibit production instability, where the productivity of the cells decreases as a function of time in culture. To counter this, we designed a passaging strategy that, rather than maximizing the time spent in log‐growth phase, mimics the first 7 days of
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Integration of just‐in‐time learning with variational autoencoder for cell culture process monitoring based on Raman spectroscopy Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-24 Mohammad Rashedi, Hamid Khodabandehlou, Tony Wang, Matthew Demers, Aditya Tulsyan, Christopher Garvin, Cenk Undey
Protein production in the biopharmaceutical industry necessitates the utilization of multiple analytical techniques and control methodologies to ensure both safety and consistency. To facilitate real‐time monitoring and control of cell culture processes, Raman spectroscopy has emerged as a versatile analytical technology. This technique, categorized as a Process Analytical Technology, employs chemometric
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Stabilization of galactose oxidase by high hydrostatic pressure: Insights on the role of cavities size Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-23 Min J. Kang, José I. Reyes‐De‐Corcuera
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Development of generic metabolic Raman calibration models using solution titration in aqueous phase and data augmentation for in-line cell culture analysis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-19 Zhijun Zhang, Zhe Lang, Gong Chen, Hang Zhou, Weichang Zhou
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Engineering of the Lrp/AsnC-type transcriptional regulator DecR as a genetically encoded biosensor for multilevel optimization of L-cysteine biosynthesis pathway in Escherichia coli Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-18 Zhiyou Zhou, Zonglin Li, Yahui Zhong, Shuai Xu, Zhimin Li
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Efficient production of l-glutathione by whole-cell catalysis with ATP regeneration from adenosine Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-17 Siqi Zuo, Jiarun Zhao, Pengfei Zhang, Wenqian Liu, Ke Bi, Peilian Wei, Jiazhang Lian, Zhinan Xu
l-glutathione (GSH) is an important tripeptide compound with extensive applications in medicine, food additives, and cosmetics industries. In this work, an innovative whole-cell catalytic strategy was developed to enhance GSH production by combining metabolic engineering of GSH biosynthetic pathways with an adenosine-based adenosine triphosphate (ATP) regeneration system in Escherichia coli. Concretely
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Biotechnology and Bioengineering: Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13
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Multivariate data analysis on multisensor measurement for inline process monitoring of adenovirus production in HEK293 cells Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Xingge Xu, Omar Farnós, Barbara C. M. F. Paes, Sean Nesdoly, Amine A. Kamen
In the era of Biopharma 4.0, process digitalization fundamentally requires accurate and timely monitoring of critical process parameters (CPPs) and quality attributes. Bioreactor systems are equipped with a variety of sensors to ensure process robustness and product quality. However, during the biphasic production of viral vectors or replication‐competent viruses for gene and cell therapies and vaccination
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Cover Image, Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Klemen Božič, Ajda Sedlar, Špela Kralj, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
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Back Cover Image, Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Yixiao Zhang, Sri Madabhushi, Tiffany Tang, Hassan Raza, David J. Busch, Xi Zhao, James Ormes, Sen Xu, Joseph Moroney, Rubin Jiang, Henry Lin, Ren Liu
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Semiautomated design and soluble expression of a chimeric antigen TbpAB01 from Glaesserella parasuis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-10 Jin‐Ping Chen, Lin Zhou, Jin‐Song Gong, Nan‐Kai Wang, Fen‐Fang Miao, Chang Su, Xin‐Le Gao, Guo‐Qiang Xu, Jin‐Song Shi, Zheng‐Hong Xu
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Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-08 Siqi Wang, Yujie Liu, Hongfu Guo, Yan Meng, Weini Xiong, Ruihua Liu, Chao Yang
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Machine learning approaches to predict TAS2R receptors for bitterants Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-08 Francesco Ferri, Marco Cannariato, Marco Agostino Deriu, Lorenzo Pallante
Bitter taste involves the detection of diverse chemical compounds by a family of G protein‐coupled receptors, known as taste receptor type 2 (TAS2R). It is often linked to toxins and harmful compounds and in particular bitter taste receptors participate in the regulation of glucose homeostasis, modulation of immune and inflammatory responses, and may have implications for various diseases. Human TAS2Rs
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Characterization and engineering of peroxisome targeting sequences for compartmentalization engineering in Pichia pastoris Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-03 Cuifang Ye, Haosen Hong, Jucan Gao, Mengxin Li, Yuanwei Gou, Di Gao, Chang Dong, Lei Huang, Zhinan Xu, Jiazhang Lian
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Biotechnology and Bioengineering: Volume 121, Number 4, April 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-31
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Plants as a cost‐effective source for customizable photosynthetic wound dressings: A proof of concept study Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-31 Sergio González‐Itier, Miguel Miranda, Rocío Corrales‐Orovio, Constanza Vera, Valentina Veloso‐Giménez, Camila Cárdenas‐Calderón, José Tomás Egaña
Oxygen is essential for tissue regeneration, playing a crucial role in several processes, including cell metabolism and immune response. Therefore, the delivery of oxygen to wounds is an active field of research, and recent studies have highlighted the potential use of photosynthetic biomaterials as alternative oxygenation approach. However, while plants have traditionally been used to enhance tissue
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Expression of filaments of the Geobacter extracellular cytochrome OmcS in Shewanella oneidensis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-31 Tong Lin, Wenqi Ding, Danni Zhang, Zixuan You, Yun Yang, Feng Li, Dake Xu, Derek R. Lovley, Hao Song
The physiological role of Geobacter sulfurreducens extracellular cytochrome filaments is a matter of debate and the development of proposed electronic device applications of cytochrome filaments awaits methods for large‐scale cytochrome nanowire production. Functional studies in G. sulfurreducens are stymied by the broad diversity of redox‐active proteins on the outer cell surface and the redundancy
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Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-29 Fangmei Liu, Kai Xia, Yuqing Chen, Ling Zhu, Lingzhi Zhu, Xuequn Zhao, Ruyi Sha, Jun Huang
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Sequence‐based design and construction of synthetic nanobody library Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-29 Chuanyong Liu, Yanping Li, Qinghua He, Jinheng Fu, Qingting Wei, Hao Lin, Ying Luo, Zhui Tu
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Development of inducible promoters for regulating gene expression in Clostridium tyrobutyricum for biobutanol production Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-28 Jun Feng, Qingke Wang, Zhen Qin, Xiaolong Guo, Hongxin Fu, Shang-Tian Yang, Jufang Wang
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Recent advances in biomedical applications of carbon and graphene quantum dots: A review Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-28 Abhijit Das, Manas Roy, Mitali Saha
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Prediction models for the flux decay profile and initial flux of microfiltration for therapeutic proteins Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-19 Kota Inoue, Yumiko Masuda, Tetsuo Torisu, Koichi Nonaka, Susumu Uchiyama
Microfiltration (MF) is an essential step during biopharmaceutical manufacturing. However, unexpected flux decay can occur. Although the flux decay profile and initial flux are important factors determining MF filterability, predicting them accurately is challenging, as the root cause of unexpected flux decay remains elusive. In this study, the methodology for developing a prediction model of flux
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Genome‐scale metabolic modeling of Thraustochytrium sp. RT2316‐16: Effects of nutrients on metabolism Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-19 Carolina Shene, Allison Leyton, Liset Flores, Daniela Chavez, Juan A. Asenjo, Yusuf Chisti
Marine thraustochytrids produce metabolically important lipids such as the long‐chain omega‐3 polyunsaturated fatty acids, carotenoids, and sterols. The growth and lipid production in thraustochytrids depends on the composition of the culture medium that often contains yeast extract as a source of amino acids. This work discusses the effects of individual amino acids provided in the culture medium
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An experimental and modeling approach to describe the deactivation of cellulases at the air–liquid interface Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-19 Laura Cachafeiro, Senta Heiss‐Blanquet, Damien Hudebine
Understanding the reaction mechanisms involved in the enzymatic hydrolysis of cellulose is important because it is kinetically the most limiting step of the bioethanol production process. The present work focuses on the enzymatic deactivation at the air–liquid interface, which is one of the aspects contributing to this global deactivation. This phenomenon has already been experimentally proven, but
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Itaconic acid production by co‐feeding of Ustilago maydis: A combined approach of experimental data, design of experiments, and metabolic modeling Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-18 Anita L. Ziegler, Lena Ullmann, Manuel Boßmann, Karla L. Stein, Ulf W. Liebal, Alexander Mitsos, Lars M. Blank
Itaconic acid is a platform chemical with a range of applications in polymer synthesis and is also discussed for biofuel production. While produced in industry from glucose or sucrose, co‐feeding of glucose and acetate was recently discussed to increase itaconic acid production by the smut fungus Ustilago maydis. In this study, we investigate the optimal co‐feeding conditions by interlocking experimental
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Residence time distribution in continuous virus filtration Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-18 Yu‐Cheng Chen, Gabriele Recanati, Fernando De Mathia, Dong‐Qiang Lin, Alois Jungbauer
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Improving stability of human three dimensional skin equivalents using plasma surface treatment Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Suphanun Phuphanitcharoenkun, Fiona Louis, Yoshihiro Sowa, Michiya Matsusaki, Tanapat Palaga
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Transport‐controlled growth decoupling for self‐induced protein expression with a glycerol‐repressible genetic circuit Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Alvaro R. Lara, Flavio Kunert, Vincent Vandenbroucke, Hilal Taymaz‐Nikerel, Luz María Martínez, Juan‐Carlos Sigala, Frank Delvigne, Guillermo Gosset, Jochen Büchs
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Continuous multi‐column capture of monoclonal antibodies with convective diffusive membrane adsorbers Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Fabian Schmitz, Elias Knöchelmann, Thomas Kruse, Mirjana Minceva, Markus Kampmann
Downstream processing is the bottleneck in the continuous manufacturing of monoclonal antibodies (mAbs). To overcome throughput limitations, two different continuous processes with a novel convective diffusive protein A membrane adsorber (MA) were investigated: the rapid cycling parallel multi‐column chromatography (RC‐PMCC) process and the rapid cycling simulated moving bed (RC‐BioSMB) process. First
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Enhancing real‐time cell culture monitoring: Automated Raman model optimization with Taguchi method Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-08 Xiaoxiao Dong, Xu Yan, Yuxiang Wan, Dong Gao, Jingyu Jiao, Haibin Wang, Haibin Qu
Raman spectroscopy has found widespread usage in monitoring cell culture processes both in research and practical applications. However, commonly, preprocessing methods, spectral regions, and modeling parameters have been chosen based on experience or trial‐and‐error strategies. These choices can significantly impact the performance of the models. There is an urgent need for a simple, effective, and
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Bringing a scalable adaptive hybrid modeling framework closer to industrial use: Application on a multiscale fungal fermentation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Thomas Rydal, Jesper Frandsen, Gisela Nadal-Rey, Mads Orla Albæk, Pedram Ramin
Digitalization has paved the way for new paradigms such as digital shadows and digital twins for fermentation processes, opening the door for real-time process monitoring, control, and optimization. With a digital shadow, real-time model adaptation to accommodate complex metabolic phenomena such as metabolic shifts of a process can be monitored. Despite the many benefits of digitalization, the potential
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Host cell protein networks as a novel co-elution mechanism during protein A chromatography Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Sherin Panikulam, Alexander Hanke, Frieder Kroener, Anette Karle, Oliver Anderka, Thomas K. Villiger, Nicolas Lebesgue
Host cell proteins (HCPs) are process-related impurities of therapeutic proteins produced in for example, Chinese hamster ovary (CHO) cells. Protein A affinity chromatography is the initial capture step to purify monoclonal antibodies or Fc-based proteins and is most effective for HCP removal. Previously proposed mechanisms that contribute to co-purification of HCPs with the therapeutic protein are
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ASIA: An automated stress‐inducible adaptor for enhanced stress protein expression in engineered Escherichia coli Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Chuan‐Chieh Hsiang, I‐Son Ng
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Platform development for high‐throughput optimization of perfusion processes—Part II: Variation of perfusion rate strategies in microwell plates Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-04 Marie Dorn, Ciara Lucas, Kerensa Klottrup‐Rees, Ken Lee, Martina Micheletti
The biopharmaceutical industry is replacing fed‐batch with perfusion processes to take advantage of reduced capital and operational costs due to the operation at high cell densities (HCD) and improved productivities. HCDs are achieved by cell retention and continuous medium exchange, which is often based on the cell‐specific perfusion rate (CSPR). To obtain a cost‐productive process the perfusion rate
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Engineering an Escherichia coli based in vivo mRNA manufacturing platform Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29 Edward Curry, George Muir, Jixin Qu, Zoltán Kis, Martyn Hulley, Adam Brown
Synthetic mRNA is currently produced in standardized in vitro transcription systems. However, this one‐size‐fits‐all approach has associated drawbacks in supply chain shortages, high reagent costs, complex product‐related impurity profiles, and limited design options for molecule‐specific optimization of product yield and quality. Herein, we describe for the first time development of an in vivo mRNA
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Correction to “Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli” Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29
Liu, A., Machas, M., Mhatre, A., Hajinajaf, N., Sarnaik, A., Nichols, N., Frazer, S., Wang, X., Varman, A. M., Nielsen, D. R. (2024). Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli. Biotechnology and Bioengineering, 121:784–794. Throughout the “Results & Discussion” section, the text “Entner-Doudoroff pathway” and the abbreviated
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Simultaneous prediction of 16 quality attributes during protein A chromatography using machine learning based Raman spectroscopy models Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-28 Jiarui Wang, Jingyi Chen, Joey Studts, Gang Wang
Several key technologies for advancing biopharmaceutical manufacturing depend on the successful implementation of process analytical technologies that can monitor multiple product quality attributes in a continuous in-line setting. Raman spectroscopy is an emerging technology in the biopharma industry that promises to fit this strategic need, yet its application is not widespread due to limited success
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Quantitative characterization of tumor cell traction force on extracellular matrix by hydrogel microsphere stress sensor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-26 Xingquan Ma, Cong Wang, Changchun Ji, Xiaoshan Cao, Yuqing Dong
Cell traction force (CTF) is a kind of active force that is a cell senses external environment and actively applies to the contact matrix which is currently a representative stress in cell–extracellular matrix (ECM) interaction. Studying the distribution and variation of CTF during cell–ECM interaction help to explain the impact of physical factors on cell behaviors from the perspective of mechanobiology
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Platform development for high‐throughput optimization of perfusion processes: Part I: Implementation of cell bleeds in microwell plates Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Marie Dorn, Kerensa Klottrup‐Rees, Ken Lee, Martina Micheletti
The promise of continuous processing to increase yields and improve product quality of biopharmaceuticals while decreasing the manufacturing footprint is transformative. Developing and optimizing perfusion operations requires screening various parameters, which is expensive and time‐consuming when using benchtop bioreactors. Scale‐down models (SDMs) are the most feasible option for high‐throughput
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NIR spectroscopy—CNN‐enabled chemometrics for multianalyte monitoring in microbial fermentation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Shantanu Banerjee, Shyamapada Mandal, Naveen G. Jesubalan, Rijul Jain, Anurag S. Rathore
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Robust platform for inline Raman monitoring and control of perfusion cell culture Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Boyong Wan, Misaal Patel, George Zhou, Michael Olma, Marco Bieri, Marvin Mueller, Emmanuel Appiah-Amponsah, Bhumit Patel, Karthik Jayapal
Perfusion cell culture has been gaining increasing popularity for biologics manufacturing due to benefits such as smaller footprint, increased productivity, consistent product quality and manufacturing flexibility, cost savings, and so forth. Process Analytics Technologies tools are highly desirable for effective monitoring and control of long-running perfusion processes. Raman has been widely investigated
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Enhanced sporulation of B. licheniformis BF-002 through automatic co-feeding of carbon and nitrogen sources Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-21 Jian Ding, Qingyuan Liu, Wenbiao Hou, Jun Cai, Bo Wang, Cheng Lu
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Biotechnology and Bioengineering: Volume 121, Number 3, March 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19
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Bayesian cell therapy process optimization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Evan Claes, Tommy Heck, Kathleen Coddens, Maarten Sonnaert, Jan Schrooten, Jan Verwaeren