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Trading acyls and swapping sugars: metabolic innovations in Solanum trichomes Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Paul D Fiesel, Rachel E Kerwin, A Daniel Jones, Robert L Last
Solanaceae (nightshade family) species synthesize a remarkable array of clade- and tissue-specific specialized metabolites. Protective acylsugars, one such class of structurally diverse metabolites, are produced by ACYLSUGAR ACYLTRANSFERASE (ASAT) enzymes from sugars and acyl-coenzyme A esters. Published research has revealed trichome acylsugars composed of glucose and sucrose cores in species across
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Isohydricity and hydraulic isolation explain reduced hydraulic failure risk in an experimental tree species mixture Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Myriam Moreno, Guillaume Simioni, Hervé Cochard, Claude Doussan, Joannès Guillemot, Renaud Decarsin, Pilar Fernandez-Conradi, Jean-Luc Dupuy, Santiago Trueba, François Pimont, Julien Ruffault, Frederic Jean, Olivier Marloie, Nicolas K Martin-StPaul
Species mixture is promoted as a crucial management option to adapt forests to climate change. However, there is little consensus on how tree diversity affects tree water stress, and the underlying mechanisms remain elusive. By using a greenhouse experiment and a soil-plant-atmosphere hydraulic model, we explored whether and why mixing the isohydric Aleppo pine (Pinus halepensis, drought avoidant)
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REDUCED CHLOROPLAST COVERAGE proteins are required for plastid proliferation and carotenoid accumulation in tomato Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Qun Hu, Hui Zhang, Yuman Song, Lijuan Song, Lingling Zhu, Hanhui Kuang, Robert M Larkin
Increasing the amount of cellular space allocated to plastids will lead to increases in the quality and yield of crop plants. However, mechanisms that allocate cellular space to plastids remain poorly understood. To test whether the tomato (Solanum lycopersicum L.) REDUCED CHLOROPLAST COVERAGE (SlREC) gene products serve as central components of the mechanism that allocates cellular space to plastids
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Molecular dissection of the pseudokinase ZED1 expands effector recognition to the tomato immune receptor ZAR1 Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Nathan Diplock, Maël Baudin, Derek Xiang, Lung-Yu Liang, Weiwen Dai, James M Murphy, Isabelle S Lucet, Jana A Hassan, Jennifer D Lewis
The highly conserved angiosperm immune receptor HOPZ-ACTIVATED RESISTANCE 1 (ZAR1) is a bacterial pathogen recognition hub that mediates resistance by guarding host kinases for modification by pathogen effectors. The pseudokinase HOPZ-ETI DEFICIENT 1 (ZED1) is the only known ZAR1-guarded protein that interacts directly with a pathogen effector, HopZ1a, from the bacterial pathogen Pseudomonas syringae
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Phased Telomere-to-Telomere Reference Genome and Pangenome Reveal an Expansion of Resistance Genes during Apple Domestication Plant Physiol. (IF 7.4) Pub Date : 2024-05-14 Ying Su, Xuanwen Yang, Yuwei Wang, Jialei Li, Qiming Long, Shuo Cao, Xu Wang, Zhenya Liu, Siyang Huang, Zhuyifu Chen, Yanling Peng, Fan Zhang, Hui Xue, Xuejing Cao, Mengyan Zhang, Gulbar Yisilam, Zhenzhou Chu, Yuan Gao, Yongfeng Zhou, Zhongjie Liu, Hua Xiao, Xinmin Tian
The cultivated apple (Malus domestica Borkh.) is a cross-pollinated perennial fruit tree of great economic importance. Previous versions of apple reference genomes were unphased, fragmented, and lacked comprehensive insights into the highly heterozygous genome, which impeded genetic studies and breeding programs in apple. In this study, we assembled a haplotype-resolved telomere-to-telomere reference
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Genotyping-by-sequencing-based high-resolution mapping reveals a single candidate gene for the grapevine veraison locus Ver1 Plant Physiol. (IF 7.4) Pub Date : 2024-05-14 Lena Frenzke, Franco Röckel, Torsten Wenke, Florian Schwander, Konrad Grützmann, Julia Naumann, Falk Zakrzewski, Tom Heinekamp, Maria Maglione, Anja Wenke, Anja Kögler, Eva Zyprian, Andreas Dahl, Franz Förster, Reinhard Töpfer, Stefan Wanke
Veraison marks the transition from berry growth to berry ripening and is a crucial phenological stage in grapevine (Vitis vinifera): the berries become soft and begin to accumulate sugars, aromatic substances, and, in red cultivars, anthocyanins for pigmentation, while the organic acid levels begin to decrease. These changes determine the potential quality of wine. However, rising global temperatures
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Transcription factor ZmEREB97 regulates nitrate uptake in maize (Zea mays) roots Plant Physiol. (IF 7.4) Pub Date : 2024-05-14 Qi Wu, Jinyan Xu, Yingdi Zhao, Yuancong Wang, Ling Zhou, Lihua Ning, Sergey Shabala, Han Zhao
Maize (Zea mays L.) has very strong requirements for nitrogen. However, the molecular mechanisms underlying the regulations of nitrogen uptake and translocation in this species are not fully understood. Here, we report that an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor ZmEREB97 functions as an important regulator in the N-signaling network in maize. Predominantly expressed and
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Modern wheat breeding selection synergistically improves above- and belowground traits Plant Physiol. (IF 7.4) Pub Date : 2024-05-13 Peng Zhao, Zihui Liu, Xue Shi, Wenyang Hou, Mingzhu Cheng, Yuxiu Liu, James Simmonds, Wanquan Ji, Cristobal Uauy, Shengbao Xu, Xiaoming Wang
The haplotypes selected by modern wheat breeding to improve aboveground traits enlarge the root systems of wheat seedlings.
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Putative rhamnogalacturonan-II glycosyltransferase identified through callus gene editing bypasses embryo lethality Plant Physiol. (IF 7.4) Pub Date : 2024-05-13 Yuan Zhang, Deepak Sharma, Yan Liang, Nick Downs, Fleur Dolman, Kristen Thorne, Ian M Black, Jose Henrique Pereira, Paul Adams, Henrik V Scheller, Malcolm O’Neill, Breeanna Urbanowicz, Jenny C Mortimer
Rhamnogalacturonan II (RG-II) is a structurally complex and conserved domain of the pectin present in the primary cell walls of vascular plants. Borate crosslinking of RG-II is required for plants to grow and develop normally. Mutations that alter RG-II structure also affect crosslinking and are lethal or severely impair growth. Thus, few genes involved in RG-II synthesis have been identified. Here
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Persulfidation and phosphorylation of transcription factor SlWRKY6 differentially regulate tomato fruit ripening Plant Physiol. (IF 7.4) Pub Date : 2024-05-10 Min Zhang, Kangdi Hu, Lin Ma, Meihui Geng, Conghe Zhang, Gaifang Yao, Hua Zhang
Cysteine desulfhydrase (LCD) catalyzes the generation of the signaling molecule hydrogen sulfide (H2S) in plants. In this study, we found that H2S can inhibit tomato (Solanum lycopersicum) fruit ripening and SlWRKY6 undergoes differential protein persulfidation in SlLCD1-overexpressing leaves. Then, further study indicated that SlWRKY6 could be persulfidated by H2S at Cys396. By construction of slwrky6
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Transcriptional Landscape and Dynamics Involved in Sugar and Acid Accumulation during Apple Fruit Development Plant Physiol. (IF 7.4) Pub Date : 2024-05-10 Baiyun Li, Lingcheng Zhu, Nanxiang Yang, Shengtao Qu, Wenjing Cao, Wenfang Ma, Xiaoyu Wei, Baiquan Ma, Fengwang Ma, Aigen Fu, Mingjun Li
In fleshy fruit, sugars and acids are central components of fruit flavor and quality. To date, the mechanisms underlying transcriptional regulation of sugar and acid during fruit development remain largely unknown. Here, we combined ATAC-seq with RNA-seq to investigate the genome-wide chromatin accessibility and to identify putative transcription factors related to sugar and acid accumulation during
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Designer transcription activator-like effectors enable discovery of cell death-inducer genes Plant Physiol. (IF 7.4) Pub Date : 2024-05-09 Roxana A Roeschlin, Sepideh M Azad, René P Grove, Ana Chuan, Lucila García, Regina Niñoles, Facundo Uviedo, Liara Villalobos-Piña, Maria E Massimino, María R Marano, Jens Boch, José Gadea
TALEs (transcription activator-like effectors) in plant-pathogenic Xanthomonas bacteria activate expression of plant genes and support infection or cause a resistance response. PthA4AT is a TALE with a particularly short DNA-binding domain harbouring only 7.5-repeats which triggers cell death in Nicotiana benthamiana; however, the genetic basis for this remains unknown. To identify possible target
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PSEUDO-RESPONSE REGULATOR 3b and transcription factor ABF3 modulate abscisic acid-dependent drought stress response in soybean Plant Physiol. (IF 7.4) Pub Date : 2024-05-08 Cong Li, Yanhang Chen, Qing Hu, Xiaolan Yang, Yunfeng Zhao, Yan Lin, Jianbo Yuan, Jinbao Gu, Yang Li, Jin He, Dong Wang, Bin Liu, Zhen-Yu Wang
The circadian system plays a pivotal role in facilitating the ability of crop plants to respond and adapt to fluctuations in their immediate environment effectively. Despite the increasing comprehension of PSEUDO-RESPONSE REGULATORs (PRRs) and their involvement in the regulation of diverse biological processes, including circadian rhythms, photoperiodic control of flowering, and responses to abiotic
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A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901 Plant Physiol. (IF 7.4) Pub Date : 2024-05-07 Angelo J Victoria, Tiago Toscano Selão, José Ángel Moreno-Cabezuelo, Lauren A Mills, Grant A R Gale, David J Lea-Smith, Alistair J McCormick
Synechococcus sp. PCC 11901 (PCC 11901) is a fast-growing marine cyanobacterial strain that has a capacity for sustained biomass accumulation to very high cell densities, comparable to that achieved by commercially relevant heterotrophic organisms. However, genetic tools to engineer PCC 11901 for biotechnology applications are limited. Here we describe a suite of tools based on the CyanoGate MoClo
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Antisense RNA regulates glutamine synthetase in a heterocyst-forming cyanobacterium Plant Physiol. (IF 7.4) Pub Date : 2024-05-06 Isidro Álvarez-Escribano, Belén Suárez-Murillo, Manuel Brenes-Álvarez, Agustín Vioque, Alicia M Muro-Pastor
Glutamine synthetase (GS) is a key enzyme involved in nitrogen assimilation and the maintenance of C/N balance, and it is strictly regulated in all bacteria. In cyanobacteria, glutamine synthetase expression is controlled by nitrogen control A (NtcA) transcription factor, which operates global nitrogen regulation in these photosynthetic organisms. Furthermore, post-translational regulation of GS is
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CO2 Response Screen in Grass Brachypodium Reveals Key Role of a MAP Kinase in CO2-Triggered Stomatal Closure Plant Physiol. (IF 7.4) Pub Date : 2024-05-06 Bryn N K Lopez, Paulo H O Ceciliato, Yohei Takahashi, Felipe J Rangel, Evana A Salem, Klara Kernig, Kelly Chow, Li Zhang, Morgana A Sidhom, Christian G Seitz, Tingwen Zheng, Richard Sibout, Debbie L Laudencia-Chingcuanco, Daniel P Woods, James Andrew McCammon, John P Vogel, Julian I Schroeder
Plants respond to increased CO2 concentrations through stomatal closure, which can contribute to increased water use efficiency. Grasses display faster stomatal responses than eudicots due to dumbbell-shaped guard cells flanked by subsidiary cells working in opposition. However, forward genetic screening for stomatal CO2 signal transduction mutants in grasses has yet to be reported. The grass model
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A maize semidwarf mutant reveals a GRAS transcription factor involved in brassinosteroid signaling Plant Physiol. (IF 7.4) Pub Date : 2024-05-06 Amanpreet Kaur, Norman B Best, Thomas Hartwig, Josh Budka, Rajdeep Khangura, Steven McKenzie, Alejandro Aragón-Raygoza, Josh Strable, Burkhard Schulz, Brian P Dilkes
Brassinosteroids (BR) and gibberellins (GA) regulate plant height and leaf angle in maize (Zea mays). Mutants with defects in BR or GA biosynthesis or signaling identify components of these pathways and enhance our knowledge about plant growth and development. In this study, we characterized three recessive mutant alleles of GRAS transcription factor 42 (gras42) in maize, a GRAS transcription factor
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Chloroplast biogenesis involves spatial coordination of nuclear and organellar gene expression in Chlamydomonas Plant Physiol. (IF 7.4) Pub Date : 2024-05-06 Yi Sun, Shiva Bakhtiari, Melissa Valente-Paterno, Yanxia Wu, Yoshiki Nishimura, Weike Shen, Christopher Law, James Dhaliwal, Daniel Dai, Khanh Huy Bui, William Zerges
The localization of translation can direct the polypeptide product to the proper intracellular compartment. Our results reveal translation by cytosolic ribosomes on a domain of the chloroplast envelope in the unicellular green alga Chlamydomonas (Chlamydomonas reinhardtii). We show that this envelope domain of isolated chloroplasts retains translationally active ribosomes and mRNAs encoding chloroplast
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Acetyl-CoA Carboxylase1 Influences ECERIFERUM2 Activity to Mediate the Synthesis of Very-Long-Chain Fatty Acid Past C28 Plant Physiol. (IF 7.4) Pub Date : 2024-05-06 Xianpeng Yang, Haodong Huang, Zhen Wang, Tegan M Haslam, Ljerka Kunst, Pingping Wang, Huayan Zhao, Shiyou Lü, Changle Ma
Cuticular wax is a protective layer on the aerial surfaces of land plants. In Arabidopsis (Arabidopsis thaliana), cuticular wax is mainly constituted of compounds derived from very-long-chain fatty acids (VLCFAs) with chain lengths longer than C28. CER2-LIKE (ECERIFERUM2-LIKE) proteins interact with CER6/KCS6 (ECERIFERUM6/β-Ketoacyl-CoA Synthase6), the key enzyme of the fatty acid elongase complex
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Auxin signaling in the cambium promotes tissue adhesion and vascular formation during Arabidopsis graft healing Plant Physiol. (IF 7.4) Pub Date : 2024-05-03 Phanu T Serivichyaswat, Abdul Kareem, Ming Feng, Charles W Melnyk
The strong ability of plants to regenerate wounds is exemplified by grafting when two plants are cut and joined together to grow as one. During graft healing, tissues attach, cells proliferate, and the vasculatures connect to form a graft union. The plant hormone auxin plays a central role, and auxin-related mutants perturb grafting success. Here, we investigated the role of individual cell types and
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40S Ribosomal Protein S6 Kinase Integrates Daylength Perception and Growth Regulation in Arabidopsis thaliana Plant Physiol. (IF 7.4) Pub Date : 2024-05-03 Marc Boix, Alba Garcia-Rodriguez, Laia Castillo, Bernat Miró, Ferga Hamilton, Sanata Tolak, Adrián Pérez, Carolina Monte-Bello, Camila Caldana, Rossana Henriques
Plant growth occurs via the interconnection of cell growth and proliferation in each organ following specific developmental and environmental cues. Therefore, different photoperiods result in distinct growth patterns due to the integration of light and circadian perception with specific Carbon (C) partitioning strategies. In addition, the TARGET OF RAPAMYCIN (TOR) kinase pathway is an ancestral signaling
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Genetically manipulated chloroplast stromal phosphate levels alter photosynthetic efficiency Plant Physiol. (IF 7.4) Pub Date : 2024-05-03 Aditi Subramani Raju, David M Kramer, Wayne K Versaw
The concentration of inorganic phosphate (Pi) in the chloroplast stroma must be maintained within narrow limits to sustain photosynthesis and to direct the partitioning of fixed carbon. However, it is unknown if these limits or the underlying contributions of different chloroplastic Pi transporters vary throughout the photoperiod or between chloroplasts in different leaf tissues. To address these questions
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Structural basis of chorismate isomerization by Arabidopsis ISOCHORISMATE SYNTHASE1 Plant Physiol. (IF 7.4) Pub Date : 2024-05-03 Zihui Su, Chengqun Niu, Sicong Zhou, Guolyu Xu, Pingchuan Zhu, Qiang Fu, Yuelin Zhang, Zhenhua Ming
Salicylic acid (SA) plays a crucial role in plant defense against biotrophic and semi-biotrophic pathogens. In Arabidopsis (Arabidopsis thaliana), isochorismate synthase 1 (AtICS1) is a key enzyme for the pathogen-induced biosynthesis of SA via catalytic conversion of chorismate into isochorismate, an essential precursor for SA synthesis. Despite the extensive knowledge of ICS1-related menaquinone
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CRISPR/Cas9-generated mutations in a sugar transporter gene reduce cassava susceptibility to bacterial blight Plant Physiol. (IF 7.4) Pub Date : 2024-05-03 Kiona Elliott, Kira M Veley, Greg Jensen, Kerrigan B Gilbert, Joanna Norton, Lukas Kambic, Marisa Yoder, Alex Weil, Sharon Motomura-Wages, Rebecca S Bart
Bacteria from the genus Xanthomonas are prolific phytopathogens that elicit disease in over 400 plant species. Xanthomonads carry a repertoire of specialized proteins called transcription activator-like (TAL) effectors that promote disease and pathogen virulence by inducing expression of host susceptibility (S) genes. Xanthomonas phaseoli pv. manihotis (Xpm) causes bacterial blight on the staple food
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Development of a mobile, high-throughput, and low-cost image-based plant growth phenotyping system Plant Physiol. (IF 7.4) Pub Date : 2024-05-02 Li’ang Yu, Hayley Sussman, Olga Khmelnitsky, Maryam Rahmati Ishka, Aparna Srinivasan, Andrew D L Nelson, Magdalena M Julkowska
Nondestructive plant phenotyping forms a key technique for unraveling molecular processes underlying plant development and response to the environment. While the emergence of high-throughput phenotyping facilities can further our understanding of plant development and stress responses, their high costs greatly hinder scientific progress. To democratize high-throughput plant phenotyping, we developed
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Transcription factors Pbr3RAV2 and PbrTTG1 regulate pear resistance to Botryosphaeria dothidea via the autophagy pathway Plant Physiol. (IF 7.4) Pub Date : 2024-05-02 Xun Sun, Fei Wang, Yun Wang, Ye Zhang, Yue Zhang, Yuting Liu, Xiaolei Sun, Kaijie Qi, Zhihua Xie, Shaoling Zhang
Pear ring rot, caused by Botryosphaeria dothidea, is the most serious disease of pear (Pyrus spp.) trees. However, the molecular mechanisms underlying pear resistance to B. dothidea remain elusive. Herein, we demonstrated that the pear AuTophagy-related Gene 1a (PbrATG1a) plays a key role in autophagic activity and resistance to B. dothidea. Stable overexpression of PbrATG1a enhanced resistance to
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Distinct types of selection and genetic architecture shape molecular variation during the domestication of vegetable crops Plant Physiol. (IF 7.4) Pub Date : 2024-04-30 Hung-Wei Chen, Chih-Cheng Chien, Cheng-Ruei Lee
Humans select vegetable crops with desirable traits via a complex evolutionary process called domestication, generating a variety of cultivars worldwide. With advances in sequencing technologies, genomic scans for “signatures of selection” are widely used to identify target loci of selection. In the early phases of domestication, humans tended to favor similar sets of phenotypes in diverse crops, resulting
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The genomic route to tomato breeding: Past, present, and future Plant Physiol. (IF 7.4) Pub Date : 2024-04-30 Yan Wang, Chuanlong Sun, Zhibiao Ye, Chuanyou Li, Sanwen Huang, Tao Lin
Over the past 10,000 years, tomato species have undergone both unintentional and intentional selection to enhance their favorable traits for human consumption and manufacturing. These selection processes have significantly influenced the genomes of tomato species and have played a critical role in improving tomato varieties. In this review, we summarize recent advances in tomato genome sequencing,
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Nucleotide-binding leucine-rich repeat receptor homologs Pib and PibH8 interact and contribute to immunity in rice Plant Physiol. (IF 7.4) Pub Date : 2024-04-30 Zhanchun Wang, Dewei Yang, Guitao Zhong, Shengping Li, Wei Wang, Dingzhong Tang
Plant nucleotide-binding leucine-rich repeat receptors (NLRs) sense pathogen effectors and activate effector-triggered immunity (ETI). Many plant NLRs form pairs with other NLRs to recognize effectors and initiate ETI. PIRICULARIA ORYZAE RESISTANCE IN BL1 (Pib), an NLR protein in rice (Oryza sativa), activates resistance by recognizing the rice blast effector AvrPib. The activation of Pib is suppressed
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Ubiquitin ligase VvPUB26 in grapevine promotes proanthocyanidin synthesis and resistance to powdery mildew Plant Physiol. (IF 7.4) Pub Date : 2024-04-30 Ting Zhao, Congbo Huang, Na Li, Yaqi Ge, Ling Wang, Yujin Tang, Yuejin Wang, Yan Li, Chaohong Zhang
Proanthocyanidins (PAs) are an important group of flavonoids that contribute to astringency, color, and flavor in grape (Vitis vinifera) and wines. They also play a crucial role in enhancing plant resistance to various stresses. However, the underlying regulatory mechanism governing PAs biosynthesis, particularly in relation to conferring resistance to powdery mildew, has not been extensively explored
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Transcription factor DIVARICATA1 positively modulates seed germination in response to salinity stress Plant Physiol. (IF 7.4) Pub Date : 2024-04-30 Da Zhang, Tan He, Xumin Wang, Chenchen Zhou, Youpeng Chen, Xin Wang, Shixiang Wang, Shuangcheng He, Yuan Guo, Zijin Liu, Mingxun Chen
Seed germination is a critical checkpoint for plant growth under unfavorable environmental conditions. In Arabidopsis (Arabidopsis thaliana), the abscisic acid (ABA) and gibberellic acid (GA) signaling pathways play important roles in modulating seed germination. However, the molecular links between salinity stress and ABA/GA signaling are not well understood. Herein, we showed that the expression
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RNA editing factor SlORRM2 regulates the formation of fruit pointed tips in tomato Plant Physiol. (IF 7.4) Pub Date : 2024-04-26 Yongfang Yang, Yajing Ji, Keru Wang, Jinyan Li, Guoning Zhu, Liqun Ma, Oren Ostersetzer-Biran, Benzhong Zhu, Daqi Fu, Guiqin Qu, Yunbo Luo, Hongliang Zhu
Domestication of tomato (Solanum lycopersicum) has led to large variation in fruit size and morphology. The development of the distal end of the fruit is a critical factor in determining its overall shape. However, the intricate mechanisms underlying distal fruit development require further exploration. This study aimed to investigate the regulatory role of an organelle RNA recognition motif (RRM)-containing
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Drought-induced circular RNAs in maize roots: separating signal from noise Plant Physiol. (IF 7.4) Pub Date : 2024-04-26 Jie Xu, Qi Wang, Xin Tang, Xiaoju Feng, Xiaoyue Zhang, Tianhong Liu, Fengkai Wu, Qingjun Wang, Xuanjun Feng, Qi Tang, Damon Lisch, Yanli Lu
Circular RNAs (CircRNAs) play an important role in diverse biological processes; however, their origin and functions, especially in plants, remain largely unclear. Here, we used two maize (Zea mays) inbred lines, as well as 14 of their derivative RILs with different drought sensitivity, to systematically characterize 8,790 circRNAs in maize roots under well-watered (WW) and water-stress (WS) conditions
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Spider mite herbivory induces an abscisic acid-driven stomatal defense Plant Physiol. (IF 7.4) Pub Date : 2024-04-26 Irene Rosa-Díaz, James Rowe, Ana Cayuela-Lopez, Vicent Arbona, Isabel Díaz, Alexander M Jones
Arthropod herbivory poses a serious threat to crop yield, prompting plants to employ intricate defense mechanisms against pest feeding. The generalist pest two-spotted spider mite (Tetranychus urticae) inflicts rapid damage and remains challenging due to its broad target range. In this study, we explored the Arabidopsis (Arabidopsis thaliana) response to T. urticae infestation, revealing the induction
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Histone H3K27 Demethylase SlJMJ3 Modulates Fruit Ripening in Tomato Plant Physiol. (IF 7.4) Pub Date : 2024-04-26 Zhiwei Li, Jing Zeng, Yijie Zhou, Xiaochun Ding, Guoxiang Jiang, Keqiang Wu, Yueming Jiang, Xuewu Duan
The histone lysine (K) demethylase 4 (KDM4/JHDM3) subfamily of jumonji domain-containing demethylases (JMJs) has been implicated in various aspects of plant development. However, their involvement in regulating the ripening of fleshy fruits remains unclear. Here, we identified SlJMJ3, a member of the KDM4/JHDM3 family, as a H3K27me3 demethylase in tomato (Solanum lycopersicum) that plays an important
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Transcription factors HB21/40/53 trigger inflorescence arrest through abscisic acid accumulation at the end of flowering Plant Physiol. (IF 7.4) Pub Date : 2024-04-26 Verónica Sánchez-Gerschon, Irene Martínez-Fernández, María R González-Bermúdez, Sergio de la Hoz-Rodríguez, Florenci V González, Jorge Lozano-Juste, Cristina Ferrándiz, Vicente Balanzà
Flowers, and hence, fruits and seeds, are produced by the activity of the inflorescence meristem after the floral transition. In plants with indeterminate inflorescences the final number of flowers produced by the inflorescence meristem is determined by the length of the flowering period, which ends with inflorescence arrest. Inflorescence arrest depends on many different factors, such as the presence
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Transcription factor CmHSFA4-CmMYBS3 complex enhances salt tolerance in chrysanthemum by repressing CmMYB121 expression Plant Physiol. (IF 7.4) Pub Date : 2024-04-26 Xinhui Wang, Yue Wang, Yuhan Jiang, Han Wang, Lijie Zhou, Fei Li, Likai Wang, Jiafu Jiang, Fadi Chen, Sumei Chen
Excessive soil salinity not only hampers plant growth and development but can also lead to plant death. Previously, we found that heat shock factor A4 (CmHSFA4) enhances the tolerance of chrysanthemum (Chrysanthemum morifolium) to salt. However, the underlying molecular mechanism remains unclear. In this study, we identified a candidate MYB transcription factor, CmMYB121, which responded to salt stress
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Celine, a long interspersed nuclear element retrotransposon, colonizes in the centromeres of poplar chromosomes Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Haoyang Xin, Yiduo Wang, Wenli Zhang, Bao Yu, Pavel Neumann, Yihang Ning, Tao Zhang, Yufeng Wu, Ning Jiang, Jiming Jiang, Mengli Xi
Centromeres in most multicellular eukaryotes are composed of long arrays of repetitive DNA sequences. Interestingly, several transposable elements, including the well-known long terminal repeat (LTR) retrotransposon CRM (centromeric retrotransposon of maize), were found to be enriched in functional centromeres marked by the centromeric histone H3 (CENH3). Here we report a centromeric long interspersed
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Co-repressors AtSDR4L and DIG1 interact with transcription factor VAL2 and promote Arabidopsis seed-to-seedling transition Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Bailan Lu, Milad Alizadeh, Ryan Hoy, Renwei Zheng, Dongeun Go, Liang Song
Two transcriptional co-repressors physically interact with a transcription factor that is known to recruit a multi-protein complex, which promotes the repression of seed maturation genes by depositing trimethylation marks on lysine 27 of the histone 3 tails.
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A group of L-type lectin receptor kinases function redundantly in mediating extracellular NAD(P) signaling in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Qi Li, Mingxi Zhou, Fiona Harris, Zhonglin Mou
An Arabidopsis mutant lacking several L-type lectin receptor kinases shows severely reduced NAD(P)-induced local and systemic immunity and biological induction of systemic acquired resistance.
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A tale of two pumps: Blue light and abscisic acid alter Arabidopsis leaf hydraulics via bundle sheath cell H + -ATPases Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Tanmayee Torne-Srivastava, Yael Grunwald, Ahan Dalal, Adi Yaaran, Menachem Moshelion, Nava Moran
The bundle sheath cell (BSC) layer tightly enveloping the xylem throughout the leaf is recognized as a major signal-perceiving “valve” in series with stomata, regulating leaf hydraulic conductance (Kleaf) and thereby radial water flow via the transpiring leaf. The BSC blue light (BL) signaling pathway increases Kleaf and the underlying BSC water permeability. Here, we explored the hypothesis that BSCs
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Imaging of labile Fe2+ and Fe3+ in living Arabidopsis thaliana roots Plant Physiol. (IF 7.4) Pub Date : 2024-04-22 Carine Alcon, Arnaud Comte, Catherine Curie, Tou Cheu Xiong
Adapting fluorescent iron imaging to living plants enables visualizing labile Fe2+ and Fe3+ pools, revealing the heterogeneous distribution of iron redox status at the tissue and cellular levels.
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Glutathionylation of a glycolytic enzyme promotes cell death and vigor loss during aging of elm seeds Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Ying Li, Yu Wang, Yu-Qi He, Tian-Tian Ye, Xu Huang, Hao Wu, Tian-Xiao Ma, Hugh W Pritchard, Xiao-Feng Wang, Hua Xue
Seed deterioration during storage is a major problem in agricultural and forestry production and for germplasm conservation. Our previous studies have shown that a mitochondrial outer membrane protein VOLTAGE-DEPENDENT ANION CHANNEL (VDAC) is involved in programmed cell death (PCD)-like viability loss during the controlled deterioration treatment (CDT) of elm (Ulmus pumila L.) seeds, but its underlying
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Jasmonate mimic modulates cell elongation by regulating antagonistic bHLH transcription factors via brassinosteroid signaling Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Xing Wang, Zhaobin Ren, Shipeng Xie, Zhaohu Li, Yuyi Zhou, Liusheng Duan
Lodging restricts growth, development, and yield formation in maize (Zea mays L.). Shorter internode length is beneficial for lodging tolerance. However, although brassinosteroids (BRs) and jasmonic acid (JA) are known to antagonistically regulate internode growth, the underlying molecular mechanism is still unclear. In this study, application of the JA mimic coronatine (COR) inhibited basal internode
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BRZ-INSENSITIVE-LONG HYPOCOTYL8 inhibits kinase-mediated phosphorylation to regulate brassinosteroid signaling Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Zhana Chagan, Genki Nakata, Shin Suzuki, Ayumi Yamagami, Ryo Tachibana, Surina Surina, Shozo Fujioka, Minami Matsui, Tetsuo Kushiro, Takuya Miyakawa, Tadao Asami, Takeshi Nakano
Glycogen synthase kinase 3 (GSK3) is an evolutionarily conserved serine/threonine protein kinase in eukaryotes. In plants, the GSK3-like kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2) functions as a central signaling node through which hormonal and environmental signals are integrated to regulate plant development and stress adaptation. BIN2 plays a major regulatory role in brassinosteroid (BR) signaling
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RNA splicing modulates the postharvest physiological deterioration of cassava storage root Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Jinbao Gu, Xiaowen Ma, Qiuxiang Ma, Zhiqiang Xia, Yan Lin, Jianbo Yuan, Yang Li, Cong Li, Yanhang Chen, Wenquan Wang, Peng Zhang, Zhen-Yu Wang
Rapid postharvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz) storage roots is a major constraint that limits the potential of this plant as a food and industrial crop. Extensive studies have been performed to explore the regulatory mechanisms underlying the PPD processes in cassava to understand their molecular and physiological responses. However, the exceptional functional
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Increased cytoplasmic Mg2+ level contributes to rice salicylic acid accumulation and broad-spectrum resistance Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Wenzhi Zeng, Yanjun Zhang, Xinyue Tian, Wenyan Li, Han Meng, Yuanchang Zhou, Zonghua Wang, Zhichang Chen, Kewei Zhang, Mo Wang
Elevating Mg2+ levels in rice cytoplasm by genetic modification or exogenous supply increases salicylic acid accumulation and enhances broad-spectrum resistance to pathogens.
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A pipeline for identification of causal mutations in barley identifies Xantha-j as the chlorophyll synthase gene Plant Physiol. (IF 7.4) Pub Date : 2024-04-17 David Stuart, Shakhira Zakhrabekova, Morten Egevang Jørgensen, Christoph Dockter, Mats Hansson
Thousands of barley (Hordeum vulgare L.) mutants have been isolated over the last century, and many are stored in gene banks across various countries. In the present work, we developed a pipeline to efficiently identify causal mutations in barley. The pipeline is also efficient for mutations located in centromeric regions. Through bulked-segregant analyses using whole genome sequencing of pooled F2
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3D reconstruction and multi-omics analysis reveal a unique pattern of embryogenesis in Ginkgo biloba Plant Physiol. (IF 7.4) Pub Date : 2024-04-17 Lingyu Ma, Zijian Hu, Weiwei Shen, Yingying Zhang, Guangchao Wang, Bang Chang, Jinkai Lu, Yaning Cui, Huimin Xu, Yun Feng, Biao Jin, Xi Zhang, Li Wang, Jinxing Lin
Ginkgo (Ginkgo biloba L.) is one of the earliest extant species in seed plant phylogeny. Embryo development patterns can provide fundamental evidence for the origin, evolution, and adaptation of seeds. However, the architectural and morphological dynamics during embryogenesis in Ginkgo biloba (G. biloba) remain elusive. Herein, we obtained over 2200 visual slices from three stages of embryo development
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BnaABF3 and BnaMYB44 regulate the transcription of zeaxanthin epoxidase genes in carotenoid and abscisic acid biosynthesis Plant Physiol. (IF 7.4) Pub Date : 2024-04-15 Shenhua Ye, Yingying Huang, Tiantian Ma, Xiaowei Ma, Rihui Li, Jinxiong Shen, Jing Wen
Zeaxanthin epoxidase (ZEP) is a key enzyme that catalyzes the conversion of zeaxanthin to violaxanthin in the carotenoid and abscisic acid (ABA) biosynthesis pathways. The rapeseed (Brassica napus) genome has 4 ZEP (BnaZEP) copies that are suspected to have undergone subfunctionalization, yet the 4 genes’ underlying regulatory mechanisms remain unknown. Here, we genetically confirmed the functional
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B-Box transcription factor BBX28 requires CONSTITUTIVE PHOTOMORPHOGENESIS1 to induce shade-avoidance response in Arabidopsis thaliana Plant Physiol. (IF 7.4) Pub Date : 2024-04-15 Maite Saura-Sánchez, Gabriel Gomez-Ocampo, Matías Ezequiel Pereyra, Carla Eliana Barraza, Andrés H Rossi, Juan P Córdoba, Javier Francisco Botto
Shade avoidance syndrome is an important adaptive strategy. Under shade, major transcriptional rearrangements underlie the reallocation of resources to elongate vegetative structures and redefine the plant architecture to compete for photosynthesis. BBX28 is a B-box transcription factor involved in seedling de-etiolation and flowering in Arabidopsis (Arabidopsis thaliana), but its function in shade-avoidance
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The trichome pattern diversity of Cardamine shares genetic mechanisms with Arabidopsis but differs in environmental drivers Plant Physiol. (IF 7.4) Pub Date : 2024-04-12 Alberto Fuster-Pons, Alba Murillo-Sánchez, Belén Méndez-Vigo, Arnald Marcer, Bjorn Pieper, Rafael Torres-Pérez, Juan Carlos Oliveros, Miltos Tsiantis, Francisco Xavier Picó, Carlos Alonso-Blanco
Natural variation in trichome pattern (amount and distribution) is prominent among populations of many angiosperms. However, the degree of parallelism in the genetic mechanisms underlying this diversity and its environmental drivers in different species remain unclear. To address these questions, we analyzed the genomic and environmental bases of leaf trichome pattern diversity in Cardamine hirsuta
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Transcription factor PgNAC72 activates DAMMARENEDIOL SYNTHASE expression to promote ginseng saponin biosynthesis Plant Physiol. (IF 7.4) Pub Date : 2024-04-12 Ting Jiang, Yue Zhang, Gege Zuo, Tiao Luo, Hui Wang, Ru Zhang, Zhiyong Luo
Ginsenosides, the primary bioactive constituents in ginseng (Panax ginseng), possess substantial pharmacological potential and are in high demand in the market. The plant hormone methyl jasmonate (MeJA) effectively elicits ginsenoside biosynthesis in P. ginseng, though the regulatory mechanism remains largely unexplored. NAC transcription factors are critical in intricate plant regulatory networks
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Direct and indirect responses of the Arabidopsis transcriptome to an induced increase in trehalose 6-phosphate Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Omri Avidan, Marina C M Martins, Regina Feil, Marc Lohse, Federico M Giorgi, Armin Schlereth, John E Lunn, Mark Stitt
Trehalose 6-phosphate (Tre6P) is an essential signal metabolite that regulates the level of sucrose, linking growth and development to the metabolic status. We hypothesized that Tre6P plays a role in mediating the regulation of gene expression by sucrose. To test this, we performed transcriptomic profiling on Arabidopsis (Arabidopsis thaliana) plants that expressed a bacterial TREHALOSE 6-PHOSPHATE
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Rice transcription factor OsWRKY37 positively regulates flowering time and grain fertility under copper deficiency Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Chenchen Ji, Haixing Li, Jingli Ding, Lu Yu, Cuncang Jiang, Chuang Wang, Sheliang Wang, Guangda Ding, Lei Shi, Fangsen Xu, Hongmei Cai
Efficient uptake, translocation, and distribution of Cu to rice (Oryza sativa) spikelets is crucial for flowering and yield production. However, the regulatory factors involved in this process remain unidentified. In this study, we isolated a WRKY transcription factor gene induced by Cu deficiency, OsWRKY37, and characterized its regulatory role in Cu uptake and transport in rice. OsWRKY37 was highly
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Multimodal imaging analysis in silver fir reveals coordination in cellulose and lignin deposition Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Gonzalo Pérez-de-Lis, Béatrice Richard, Fabienne Quilès, Aurélie Deveau, Ignatius-Kristia Adikurnia, Cyrille B K Rathgeber
Despite lignin being a key component of wood, the dynamics of tracheid lignification are generally overlooked in xylogenesis studies, which hampers our understanding of environmental drivers and blurs the interpretation of isotopic and anatomical signals stored in tree rings. Here, we analyzed cell wall formation in silver fir (Abies alba Mill.) tracheids to determine if cell wall lignification lags
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Photosynthetic capacity and assimilate transport of the lower canopy influence maize yield under high planting density Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Yanyan Yan, Fengying Duan, Xia Li, Rulang Zhao, Peng Hou, Ming Zhao, Shaokun Li, Yonghong Wang, Tingbo Dai, Wenbin Zhou
Photosynthesis is a major trait of interest for development of high-yield crop plants. However, little is known about the effects of high-density planting on photosynthetic responses at the whole-canopy level. Using the high-yielding maize (Zea mays L.) cultivars ‘LY66’, ‘MC670’, and ‘JK968’, we here conducted a two-year field experiment to assess ear development in addition to leaf characteristics
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Age-dependent changes in leaf size in apple are governed by a cytokinin-integrated module Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Xumei Jia, Shuo Xu, Yuting Wang, Lu Jin, Tengteng Gao, Zhijun Zhang, Chao Yang, Yubin Qing, Chao Li, Fengwang Ma
Plants undergo various age-dependent changes in leaf morphology during the transition from the juvenile to the adult stage. However, the precise molecular mechanisms governing these changes in apple (Malus domestica) remain unknown. Here, we showed that CYTOKININ OXIDASE/DEHYDROGENASE5 (MdCKX5), an age-dependent gene, encodes a functional CKX enzyme and serves as the common downstream target of SQUAMOSA
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Global dynamics and cytokinin participation of salt gland development trajectory in recretohalophyte Limonium bicolor Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Boqing Zhao, Yaru Gao, Qiuyu Ma, Xi Wang, Jian-Kang Zhu, Weiqiang Li, Baoshan Wang, Fang Yuan
Salt gland is an epidermal Na+ secretory structure that enhances salt resistance in the recretohalophyte sea lavender (Limonium bicolor). To elucidate the salt gland development trajectory and related molecular mechanisms, we performed single-cell RNA sequencing of L. bicolor protoplasts from young leaves at salt gland initiation and differentiation stages. Dimensionality reduction analyses defined
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The CELL NUMBER REGULATOR FW2.2 protein regulates cell-to-cell communication in tomato by modulating callose deposition at plasmodesmata Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Arthur Beauchet, Norbert Bollier, Magali Grison, Valérie Rofidal, Frédéric Gévaudant, Emmanuelle Bayer, Nathalie Gonzalez, Christian Chevalier
FW2.2 (standing for FRUIT WEIGHT 2.2), the founding member of the CELL NUMBER REGULATOR (CNR) gene family, was the first cloned gene underlying a quantitative trait locus (QTL) governing fruit size and weight in tomato (Solanum lycopersicum). However, despite this discovery over 20 years ago, the molecular mechanisms by which FW2.2 negatively regulates cell division during fruit growth remain undeciphered