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Elmo meets Quasimodo Nat. Plants (IF 18.0) Pub Date : 2024-05-16 Raphael Trösch
Pectin of middle lamellae consists predominantly of homogalacturonan. Known enzymes in homogalacturonan biosynthesis are the galacuronosyl transferase QUASIMODO 1 (QUA1), the pectin methyltransferase QUASIMODO 2 (QUA2) and GALACTURONOSYLTRANSFERASE 9 (GAUT9), which is a homolog of QUA1. The qua mutants have a dwarf phenotype and reduced cell adhesion due to defective middle lamellae, while the gaut9
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Plant height as an indicator for alpine carbon sequestration and ecosystem response to warming Nat. Plants (IF 18.0) Pub Date : 2024-05-16 Quan Quan, Nianpeng He, Ruiyang Zhang, Jinsong Wang, Yiqi Luo, Fangfang Ma, Junxiao Pan, Ruomeng Wang, Congcong Liu, Jiahui Zhang, Yiheng Wang, Bing Song, Zhaolei Li, Qingping Zhou, Guirui Yu, Shuli Niu
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Trading acyls and swapping sugars: metabolic innovations in Solanum trichomes Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Paul D Fiesel, Rachel E Kerwin, A Daniel Jones, Robert L Last
Solanaceae (nightshade family) species synthesize a remarkable array of clade- and tissue-specific specialized metabolites. Protective acylsugars, one such class of structurally diverse metabolites, are produced by ACYLSUGAR ACYLTRANSFERASE (ASAT) enzymes from sugars and acyl-coenzyme A esters. Published research has revealed trichome acylsugars composed of glucose and sucrose cores in species across
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Isohydricity and hydraulic isolation explain reduced hydraulic failure risk in an experimental tree species mixture Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Myriam Moreno, Guillaume Simioni, Hervé Cochard, Claude Doussan, Joannès Guillemot, Renaud Decarsin, Pilar Fernandez-Conradi, Jean-Luc Dupuy, Santiago Trueba, François Pimont, Julien Ruffault, Frederic Jean, Olivier Marloie, Nicolas K Martin-StPaul
Species mixture is promoted as a crucial management option to adapt forests to climate change. However, there is little consensus on how tree diversity affects tree water stress, and the underlying mechanisms remain elusive. By using a greenhouse experiment and a soil-plant-atmosphere hydraulic model, we explored whether and why mixing the isohydric Aleppo pine (Pinus halepensis, drought avoidant)
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REDUCED CHLOROPLAST COVERAGE proteins are required for plastid proliferation and carotenoid accumulation in tomato Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Qun Hu, Hui Zhang, Yuman Song, Lijuan Song, Lingling Zhu, Hanhui Kuang, Robert M Larkin
Increasing the amount of cellular space allocated to plastids will lead to increases in the quality and yield of crop plants. However, mechanisms that allocate cellular space to plastids remain poorly understood. To test whether the tomato (Solanum lycopersicum L.) REDUCED CHLOROPLAST COVERAGE (SlREC) gene products serve as central components of the mechanism that allocates cellular space to plastids
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Molecular dissection of the pseudokinase ZED1 expands effector recognition to the tomato immune receptor ZAR1 Plant Physiol. (IF 7.4) Pub Date : 2024-05-15 Nathan Diplock, Maël Baudin, Derek Xiang, Lung-Yu Liang, Weiwen Dai, James M Murphy, Isabelle S Lucet, Jana A Hassan, Jennifer D Lewis
The highly conserved angiosperm immune receptor HOPZ-ACTIVATED RESISTANCE 1 (ZAR1) is a bacterial pathogen recognition hub that mediates resistance by guarding host kinases for modification by pathogen effectors. The pseudokinase HOPZ-ETI DEFICIENT 1 (ZED1) is the only known ZAR1-guarded protein that interacts directly with a pathogen effector, HopZ1a, from the bacterial pathogen Pseudomonas syringae
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A spatial transcriptome map of the developing maize ear Nat. Plants (IF 18.0) Pub Date : 2024-05-14 Yuebin Wang, Yun Luo, Xing Guo, Yunfu Li, Jiali Yan, Wenwen Shao, Wenjie Wei, Xiaofeng Wei, Tao Yang, Jing Chen, Lihua Chen, Qian Ding, Minji Bai, Lin Zhuo, Li Li, David Jackson, Zuxin Zhang, Xun Xu, Jianbing Yan, Huan Liu, Lei Liu, Ning Yang
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Evolutionary relationship of moso bamboo forms and a multihormone regulatory cascade involving culm shape variation Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-14 Yan Liu, Chenglei Zhu, Xianghua Yue, Zeming Lin, Hui Li, Xiaolin Di, Jiangfei Wang, Zhimin Gao
SummaryMoso bamboo (Phyllostachys edulis) known as Mao Zhu (MZ) in Chinese exhibits various forms with distinct morphological characteristics. However, the evolutionary relationship among MZ forms and the mechanisms of culm shape variation are still lacking. Here, the main differences among MZ forms were identified as culm shape variation, which were confirmed by analysing MZ forms (799 bamboo culms)
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The complex relationship between disease resistance and yield in crops Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-14 Mark C. Derbyshire, Toby E. Newman, William J. W. Thomas, Jacqueline Batley, David Edwards
SummaryIn plants, growth and defence are controlled by many molecular pathways that are antagonistic to one another. This results in a ‘growth‐defence trade‐off’, where plants temporarily reduce growth in response to pests or diseases. Due to this antagonism, genetic variants that improve resistance often reduce growth and vice versa. Therefore, in natural populations, the most disease resistant individuals
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Trees adjust nutrient acquisition strategies across tropical forest secondary succession New Phytol. (IF 9.4) Pub Date : 2024-05-14 Michelle Y. Wong, Nina Wurzburger, Jefferson S. Hall, S. Joseph Wright, Wenguang Tang, Lars O. Hedin, Kristin Saltonstall, Michiel van Breugel, Sarah A. Batterman
Summary Nutrient limitation may constrain the ability of recovering and mature tropical forests to serve as a carbon sink. However, it is unclear to what extent trees can utilize nutrient acquisition strategies – especially root phosphatase enzymes and mycorrhizal symbioses – to overcome low nutrient availability across secondary succession. Using a large‐scale, full factorial nitrogen and phosphorus
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LbSakA‐mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development New Phytol. (IF 9.4) Pub Date : 2024-05-14 Liang Shi, Zi Wang, Ju Hong Chen, Hao Qiu, Wei Dong Liu, Xiao Yan Zhang, Francis M. Martin, Ming Wen Zhao
Summary Ectomycorrhizal symbiosis, which involves mutually beneficial interactions between soil fungi and tree roots, is essential for promoting tree growth. To establish this symbiotic relationship, fungal symbionts must initiate and sustain mutualistic interactions with host plants while avoiding host defense responses. This study investigated the role of reactive oxygen species (ROS) generated by
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Shifts in mycorrhizal types of fungi and plants in response to fertilisation, warming and herbivory in a tundra grassland New Phytol. (IF 9.4) Pub Date : 2024-05-14 Coline Le Noir de Carlan, Elina Kaarlejärvi, Caroline De Tender, Thilo Heinecke, Anu Eskelinen, Erik Verbruggen
Summary Climate warming is severely affecting high‐latitude regions. In the Arctic tundra, it may lead to enhanced soil nutrient availability and interact with simultaneous changes in grazing pressure. It is presently unknown how these concurrently occurring global change drivers affect the root‐associated fungal communities, particularly mycorrhizal fungi, and whether changes coincide with shifts
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ATR2Cala2 from Arabidopsis‐infecting downy mildew requires 4 TIR‐NLR immune receptors for full recognition New Phytol. (IF 9.4) Pub Date : 2024-05-14 Dae Sung Kim, Yufei Li, Hee‐Kyung Ahn, Alison Woods‐Tör, Volkan Cevik, Oliver J. Furzer, Wenbo Ma, Mahmut Tör, Jonathan D. G. Jones
Summary Arabidopsis Col‐0 RPP2A and RPP2B confer recognition of Arabidopsis downy mildew (Hyaloperonospora arabidopsidis [Hpa]) isolate Cala2, but the identity of the recognized ATR2Cala2 effector was unknown. To reveal ATR2Cala2, an F2 population was generated from a cross between Hpa‐Cala2 and Hpa‐Noks1. We identified ATR2Cala2 as a non‐canonical RxLR‐type effector that carries a signal peptide,
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Row1, a member of a new family of conserved fungal proteins involved in infection, is required for appressoria functionality in Ustilago maydis New Phytol. (IF 9.4) Pub Date : 2024-05-14 María Dolores Pejenaute‐Ochoa, Laura Tomás‐Gallardo, José I. Ibeas, Ramón R. Barrales
Summary The appressorium of phytopathogenic fungi is a specific structure with a crucial role in plant cuticle penetration. Pathogens with melanized appressoria break the cuticle through cell wall melanization and intracellular turgor pressure. However, in fungi with nonmelanized appressorium, the mechanisms governing cuticle penetration are poorly understood. Here we characterize Row1, a previously
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Phased Telomere-to-Telomere Reference Genome and Pangenome Reveal an Expansion of Resistance Genes during Apple Domestication Plant Physiol. (IF 7.4) Pub Date : 2024-05-14 Ying Su, Xuanwen Yang, Yuwei Wang, Jialei Li, Qiming Long, Shuo Cao, Xu Wang, Zhenya Liu, Siyang Huang, Zhuyifu Chen, Yanling Peng, Fan Zhang, Hui Xue, Xuejing Cao, Mengyan Zhang, Gulbar Yisilam, Zhenzhou Chu, Yuan Gao, Yongfeng Zhou, Zhongjie Liu, Hua Xiao, Xinmin Tian
The cultivated apple (Malus domestica Borkh.) is a cross-pollinated perennial fruit tree of great economic importance. Previous versions of apple reference genomes were unphased, fragmented, and lacked comprehensive insights into the highly heterozygous genome, which impeded genetic studies and breeding programs in apple. In this study, we assembled a haplotype-resolved telomere-to-telomere reference
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Genotyping-by-sequencing-based high-resolution mapping reveals a single candidate gene for the grapevine veraison locus Ver1 Plant Physiol. (IF 7.4) Pub Date : 2024-05-14 Lena Frenzke, Franco Röckel, Torsten Wenke, Florian Schwander, Konrad Grützmann, Julia Naumann, Falk Zakrzewski, Tom Heinekamp, Maria Maglione, Anja Wenke, Anja Kögler, Eva Zyprian, Andreas Dahl, Franz Förster, Reinhard Töpfer, Stefan Wanke
Veraison marks the transition from berry growth to berry ripening and is a crucial phenological stage in grapevine (Vitis vinifera): the berries become soft and begin to accumulate sugars, aromatic substances, and, in red cultivars, anthocyanins for pigmentation, while the organic acid levels begin to decrease. These changes determine the potential quality of wine. However, rising global temperatures
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Transcription factor ZmEREB97 regulates nitrate uptake in maize (Zea mays) roots Plant Physiol. (IF 7.4) Pub Date : 2024-05-14 Qi Wu, Jinyan Xu, Yingdi Zhao, Yuancong Wang, Ling Zhou, Lihua Ning, Sergey Shabala, Han Zhao
Maize (Zea mays L.) has very strong requirements for nitrogen. However, the molecular mechanisms underlying the regulations of nitrogen uptake and translocation in this species are not fully understood. Here, we report that an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor ZmEREB97 functions as an important regulator in the N-signaling network in maize. Predominantly expressed and
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Molecular-level carbon traits underlie the multidimensional fine root economics space Nat. Plants (IF 18.0) Pub Date : 2024-05-13 Mengke Wang, Deliang Kong, Xiaohan Mo, Yinghui Wang, Qingpei Yang, Paul Kardol, Oscar J. Valverde-Barrantes, Myrna J. Simpson, Hui Zeng, Peter B. Reich, Joana Bergmann, Nishanth Tharayil, Junjian Wang
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Dynamic Arabidopsis P5CS filament facilitates substrate channelling Nat. Plants (IF 18.0) Pub Date : 2024-05-13 Chen-Jun Guo, Tianyi Zhang, Qingqing Leng, Xian Zhou, Jiale Zhong, Ji-Long Liu
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Alternative electron pathways of photosynthesis power green algal CO2 capture Plant Cell (IF 11.6) Pub Date : 2024-05-13 Gilles Peltier, Carolyne Stoffel, Justin Findinier, Sai Kiran Madireddi, Ousmane Dao, Virginie Epting, Amélie Morin, Arthur Grossman, Yonghua Li-Beisson, Adrien Burlacot
Microalgae contribute to about half of global net photosynthesis, which converts sunlight into the chemical energy (ATP and NADPH) used to transform CO2 into biomass. Alternative electron pathways of photosynthesis have been proposed to generate additional ATP that is required to sustain CO2 fixation. However, the relative importance of each alternative pathway remains elusive. Here, we dissect and
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A transthyretin-like protein acts downstream of miR397 and LACCASE to regulate grain yield in rice Plant Cell (IF 11.6) Pub Date : 2024-05-13 Yang Yu, Rui-Rui He, Lu Yang, Yan-Zhao Feng, Jiao Xue, Qing Liu, Yan-Fei Zhou, Meng-Qi Lei, Yu-Chan Zhang, Jian-Ping Lian, Yue-Qin Chen
Increasing grain yield is a major goal of breeders due to the rising global demand for food. We previously reported that the miR397–LACCASE (OsLAC) module regulates brassinosteroid (BR) signaling and grain yield in rice (Oryza sativa). However, the precise roles of laccase enzymes in the BR pathway remain unclear. Here, we report that OsLAC controls grain yield by preventing the turnover of TRANSTHYRETIN-LIKE
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The stomatal response to vapor pressure deficit drives the apparent temperature response of photosynthesis in tropical forests New Phytol. (IF 9.4) Pub Date : 2024-05-13 Martijn Slot, Sami W. Rifai, Chinedu E. Eze, Klaus Winter
Summary As temperature rises, net carbon uptake in tropical forests decreases, but the underlying mechanisms are not well understood. High temperatures can limit photosynthesis directly, for example by reducing biochemical capacity, or indirectly through rising vapor pressure deficit (VPD) causing stomatal closure. To explore the independent effects of temperature and VPD on photosynthesis we analyzed
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Skeletonema marinoi ecotypes show specific habitat‐related responses to fluctuating light supporting high potential for growth under photobioreactor light regime New Phytol. (IF 9.4) Pub Date : 2024-05-13 Charlotte Volpe, Marianne Nymark, Tom Andersen, Per Winge, Johann Lavaud, Olav Vadstein
Summary Diatoms are a diverse group of phytoplankton usually dominating areas characterized by rapidly shifting light conditions. Because of their high growth rates and interesting biochemical profile, their biomass is considered for various commercial applications. This study aimed at identifying strains with superior growth in a photobioreactor (PBR) by screening the natural intraspecific diversity
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Soil microbial identity explains home‐field advantage for litter decomposition New Phytol. (IF 9.4) Pub Date : 2024-05-13 Nobuhiko Shigyo, Kiyoshi Umeki, Toshihide Hirao
Summary Unraveling the mechanisms of home‐field advantage (HFA) is essential to gain a complete understanding of litter decomposition processes. However, knowledge of the relationships between HFA effects and microbial communities is lacking. To examine HFA effects on litter decomposition, we identified the microbial communities and conducted a reciprocal transplant experiment, including all possible
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Modern wheat breeding selection synergistically improves above- and belowground traits Plant Physiol. (IF 7.4) Pub Date : 2024-05-13 Peng Zhao, Zihui Liu, Xue Shi, Wenyang Hou, Mingzhu Cheng, Yuxiu Liu, James Simmonds, Wanquan Ji, Cristobal Uauy, Shengbao Xu, Xiaoming Wang
The haplotypes selected by modern wheat breeding to improve aboveground traits enlarge the root systems of wheat seedlings.
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Putative rhamnogalacturonan-II glycosyltransferase identified through callus gene editing bypasses embryo lethality Plant Physiol. (IF 7.4) Pub Date : 2024-05-13 Yuan Zhang, Deepak Sharma, Yan Liang, Nick Downs, Fleur Dolman, Kristen Thorne, Ian M Black, Jose Henrique Pereira, Paul Adams, Henrik V Scheller, Malcolm O’Neill, Breeanna Urbanowicz, Jenny C Mortimer
Rhamnogalacturonan II (RG-II) is a structurally complex and conserved domain of the pectin present in the primary cell walls of vascular plants. Borate crosslinking of RG-II is required for plants to grow and develop normally. Mutations that alter RG-II structure also affect crosslinking and are lethal or severely impair growth. Thus, few genes involved in RG-II synthesis have been identified. Here
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From trade‐off to synergy: microbial insights into enhancing plant growth and immunity Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-12 Yee‐Shan Ku, Yi‐Jun Liao, Shian‐Peng Chiou, Hon‐Ming Lam, Ching Chan
SummaryThe reduction in crop yield caused by pathogens and pests presents a significant challenge to global food security. Genetic engineering, which aims to bolster plant defence mechanisms, emerges as a cost‐effective solution for disease control. However, this approach often incurs a growth penalty, known as the growth‐defence trade‐off. The precise molecular mechanisms governing this phenomenon
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Gland‐specific GhVQ22 negatively regulates gland size and affects secondary metabolic accumulation in cotton Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-11 Peng‐Bao Wang, Xiang‐Fei Cheng, Peng‐Yu Wang, Xiao‐Lin Zhao, Lu Liu, Cheng‐De Yu, Roshan Zameer, Zhi‐Fang Li, Chun‐Peng Song, Chang‐Song Zou
Cotton (Gossypium spp.) has evolved pigment glands (PGs) that accumulate toxic terpenoids, such as gossypol, which serve as a defence mechanism against pests (Gadelha et al., 2014). Laboratory experiments and field trials have confirmed that PGs are essential for tolerance to chewing pests in cotton (Benedict et al., 1977; Mao et al., 2007). Altering the ability of PGs to synthesize and accumulate
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Protein complexes from edible mushrooms as a sustainable potato protection against coleopteran pests Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-11 Karmen Pogačar, Maja Grundner, Primož Žigon, Anna Coll, Anastasija Panevska, Tjaša Lukan, Marko Petek, Jaka Razinger, Kristina Gruden, Kristina Sepčić
SummaryProtein complexes from edible oyster mushrooms (Pleurotus sp.) composed of pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) exert toxicity in feeding tests against Colorado potato beetle (CPB) larvae, acting through the interaction with insect‐specific membrane sphingolipid. Here we present a new strategy for crop protection, based on in planta production of PlyA2/PlyB protein complexes,
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OsEIL2 balances rice immune responses against (hemi)biotrophic and necrotrophic pathogens via the salicylic acid and jasmonic acid synergism New Phytol. (IF 9.4) Pub Date : 2024-05-11 Yudan Zhao, Xiaoying Zhu, Cheng‐Min Shi, Guojuan Xu, Shimin Zuo, Yanlong Shi, Wenlei Cao, Houxiang Kang, Wende Liu, Ruyi Wang, Yuese Ning, Guo‐Liang Wang, Xuli Wang
Summary Plants typically activate distinct defense pathways against various pathogens. Heightened resistance to one pathogen often coincides with increased susceptibility to another pathogen. However, the underlying molecular basis of this antagonistic response remains unclear. Here, we demonstrate that mutants defective in the transcription factor ETHYLENE‐INSENSITIVE 3‐LIKE 2 (OsEIL2) exhibited enhanced
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Lipid turnover through lipophagy in the newly identified extremophilic green microalga Chlamydomonas urium New Phytol. (IF 9.4) Pub Date : 2024-05-11 María Esther Pérez‐Pérez, Manuel J. Mallén‐Ponce, Yosu Odriozola‐Gil, Alejandro Rubio, Joaquín J. Salas, Enrique Martínez‐Force, Antonio J. Pérez‐Pulido, José L. Crespo
Summary Autophagy is a central degradative pathway highly conserved among eukaryotes, including microalgae, which remains unexplored in extremophilic organisms. In this study, we described and characterized autophagy in the newly identified extremophilic green microalga Chlamydomonas urium, which was isolated from an acidic environment. The nuclear genome of C. urium was sequenced, assembled and annotated
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AvrSr27 is a zinc‐bound effector with a modular structure important for immune recognition New Phytol. (IF 9.4) Pub Date : 2024-05-11 Megan A. Outram, Jian Chen, Sean Broderick, Zhao Li, Shouvik Aditya, Nuren Tasneem, Taj Arndell, Cheryl Blundell, Daniel J. Ericsson, Melania Figueroa, Jana Sperschneider, Peter N. Dodds, Simon J. Williams
Summary Effector proteins are central to the success of plant pathogens, while immunity in host plants is driven by receptor‐mediated recognition of these effectors. Understanding the molecular details of effector–receptor interactions is key for the engineering of novel immune receptors. Here, we experimentally determined the crystal structure of the Puccinia graminis f. sp. tritici (Pgt) effector
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Dynamic regulation of water potential in Juniperus osteosperma mediates ecosystem carbon fluxes New Phytol. (IF 9.4) Pub Date : 2024-05-10 Jessica S. Guo, Mallory L. Barnes, William K. Smith, William R. L. Anderegg, Steven A. Kannenberg
Summary Some plants exhibit dynamic hydraulic regulation, in which the strictness of hydraulic regulation (i.e. iso/anisohydry) changes in response to environmental conditions. However, the environmental controls over iso/anisohydry and the implications of flexible hydraulic regulation for plant productivity remain unknown. In Juniperus osteosperma, a drought‐resistant dryland conifer, we collected
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Complex petal spot formation in the Beetle Daisy (Gorteria diffusa) relies on spot‐specific accumulation of malonylated anthocyanin regulated by paralogous GdMYBSG6 transcription factors New Phytol. (IF 9.4) Pub Date : 2024-05-10 Róisín Fattorini, Farahnoz N. Khojayori, Gregory Mellers, Edwige Moyroud, Eva Herrero, Roman T. Kellenberger, Rachel Walker, Qi Wang, Lionel Hill, Beverley J. Glover
Summary Gorteria diffusa has elaborate petal spots that attract pollinators through sexual deception, but how G. diffusa controls spot development is largely unknown. Here, we investigate how pigmentation is regulated during spot formation. We determined the anthocyanin composition of G. diffusa petals and combined gene expression analysis with protein interaction assays to characterise R2R3‐MYBs that
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GLR‐dependent calcium and electrical signals are not coupled to systemic, oxylipin‐based wound‐induced gene expression in Marchantia polymorpha New Phytol. (IF 9.4) Pub Date : 2024-05-10 Maite Sanmartín, Enrique Rojo, Andrzej Kurenda, Beatriz Larruy‐García, Ángel M. Zamarreño, M. Otilia Delgadillo, Pavel Brito‐Gutiérrez, José M. García‐Mina, Edward E. Farmer, Jose J. Sánchez‐Serrano
Summary In angiosperms, wound‐derived signals travel through the vasculature to systemically activate defence responses throughout the plant. In Arabidopsis thaliana, activity of vasculature‐specific Clade 3 glutamate receptor‐like (GLR) channels is required for the transmission of electrical signals and cytosolic Ca2+ ([Ca2+]cyt) waves from wounded leaves to distal tissues, triggering activation of
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Joint evolution of mutualistic interactions, pollination, seed dispersal mutualism, and mycorrhizal symbiosis in trees New Phytol. (IF 9.4) Pub Date : 2024-05-10 Akira Yamawo, Misuzu Ohno
Summary Mycorrhizal symbiosis, seed dispersal, and pollination are recognized as the most prominent mutualistic interactions in terrestrial ecosystems. However, it remains unclear how these symbiotic relationships have interacted to contribute to current plant diversity. We analyzed evolutionary relationships among mycorrhizal type, seed dispersal mode, and pollination mode in two global databases
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Persulfidation and phosphorylation of transcription factor SlWRKY6 differentially regulate tomato fruit ripening Plant Physiol. (IF 7.4) Pub Date : 2024-05-10 Min Zhang, Kangdi Hu, Lin Ma, Meihui Geng, Conghe Zhang, Gaifang Yao, Hua Zhang
Cysteine desulfhydrase (LCD) catalyzes the generation of the signaling molecule hydrogen sulfide (H2S) in plants. In this study, we found that H2S can inhibit tomato (Solanum lycopersicum) fruit ripening and SlWRKY6 undergoes differential protein persulfidation in SlLCD1-overexpressing leaves. Then, further study indicated that SlWRKY6 could be persulfidated by H2S at Cys396. By construction of slwrky6
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Transcriptional Landscape and Dynamics Involved in Sugar and Acid Accumulation during Apple Fruit Development Plant Physiol. (IF 7.4) Pub Date : 2024-05-10 Baiyun Li, Lingcheng Zhu, Nanxiang Yang, Shengtao Qu, Wenjing Cao, Wenfang Ma, Xiaoyu Wei, Baiquan Ma, Fengwang Ma, Aigen Fu, Mingjun Li
In fleshy fruit, sugars and acids are central components of fruit flavor and quality. To date, the mechanisms underlying transcriptional regulation of sugar and acid during fruit development remain largely unknown. Here, we combined ATAC-seq with RNA-seq to investigate the genome-wide chromatin accessibility and to identify putative transcription factors related to sugar and acid accumulation during
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Genetically optimizing soybean nodulation improves yield and protein content Nat. Plants (IF 18.0) Pub Date : 2024-05-09 Xiangbin Zhong, Jie Wang, Xiaolei Shi, Mengyan Bai, Cuicui Yuan, Chenlin Cai, Nan Wang, Xiaomin Zhu, Huaqin Kuang, Xin Wang, Jiaqing Su, Xin He, Xiao Liu, Wenqiang Yang, Chunyan Yang, Fanjiang Kong, Ertao Wang, Yuefeng Guan
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The OsMOB1A–OsSTK38 kinase complex phosphorylates CYCLIN C, controlling grain size and weight in rice Plant Cell (IF 11.6) Pub Date : 2024-05-09 Guoxin Chen, Jiabei Gao, Suting Wu, Yuan Chang, Zhenhua Chen, Jing Sun, Liying Zhang, Jinxia Wu, Xuehui Sun, William Paul Quick, Xuean Cui, Zhiguo Zhang, Tiegang Lu
Grain size and weight are crucial yield-related traits in rice (Oryza sativa). Although certain key genes associated with rice grain size and weight have been successfully cloned, the molecular mechanisms underlying grain size and weight regulation remain elusive. Here, we identified a molecular pathway regulating grain size and weight in rice involving the MPS ONE BINDER KINASE ACTIVATOR-LIKE 1A–
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The microRNA408–plantacyanin module balances plant growth and drought resistance by regulating reactive oxygen species homeostasis in guard cells Plant Cell (IF 11.6) Pub Date : 2024-05-09 Yanzhi Yang, Lei Xu, Chen Hao, Miaomiao Wan, Yihan Tao, Yan Zhuang, Yanning Su, Lei Li
The conserved microRNA (miRNA) miR408 enhances photosynthesis and compromises stress tolerance in multiple plants, but the cellular mechanism underlying its function remains largely unclear. Here, we show that in Arabidopsis (Arabidopsis thaliana), the transcript encoding the blue copper protein PLANTACYANIN (PCY) is the primary target for miR408 in vegetative tissues. PCY is preferentially expressed
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Proteolysis in plant immunity Plant Cell (IF 11.6) Pub Date : 2024-05-09 Yanan Liu, Edan Jackson, Xueru Liu, Xingchuan Huang, Renier A L van der Hoorn, Yuelin Zhang, Xin Li
Compared with transcription and translation, protein degradation machineries can act faster and be targeted to different subcellular compartments, enabling immediate regulation of signaling events. It is therefore not surprising that proteolysis has been used extensively to control homeostasis of key regulators in different biological processes and pathways. Over the past decades, numerous studies
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A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis Plant Cell (IF 11.6) Pub Date : 2024-05-09 Julia Legen, Benjamin Lenzen, Nitin Kachariya, Stephanie Feltgen, Yang Gao, Simon Mergenthal, Willi Weber, Enrico Klotzsch, Reimo Zoschke, Michael Sattler, Christian Schmitz-Linneweber
Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like
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Phyllosphere fungal diversity generates pervasive nonadditive effects on plant performance New Phytol. (IF 9.4) Pub Date : 2024-05-09 Brianna K. Almeida, Elan H. Tran, Michelle E. Afkhami
Summary Plants naturally harbor diverse microbiomes that can dramatically impact their health and productivity. However, it remains unclear how fungal microbiome diversity, especially in the phyllosphere, impacts intermicrobial interactions and consequent nonadditive effects on plant productivity. Combining manipulative experiments, field collections, culturing, microbiome sequencing, and synthetic
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Designer transcription activator-like effectors enable discovery of cell death-inducer genes Plant Physiol. (IF 7.4) Pub Date : 2024-05-09 Roxana A Roeschlin, Sepideh M Azad, René P Grove, Ana Chuan, Lucila García, Regina Niñoles, Facundo Uviedo, Liara Villalobos-Piña, Maria E Massimino, María R Marano, Jens Boch, José Gadea
TALEs (transcription activator-like effectors) in plant-pathogenic Xanthomonas bacteria activate expression of plant genes and support infection or cause a resistance response. PthA4AT is a TALE with a particularly short DNA-binding domain harbouring only 7.5-repeats which triggers cell death in Nicotiana benthamiana; however, the genetic basis for this remains unknown. To identify possible target
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MicroRNA169 integrates multiple factors to modulate plant growth and abiotic stress responses Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-08 Xiaotong Chen, Zhaohui Chen, Andrew Fiorentino, Morgan Kuess, Nishanth Tharayil, Rohit Kumar, Elizabeth Leonard, Rooksana Noorai, Qian Hu, Hong Luo
SummaryMicroRNA169 (miR169) has been implicated in multi‐stress regulation in annual species such as Arabidopsis, maize and rice. However, there is a lack of experimental functional and mechanistic studies of miR169 in plants, especially in perennial species, and its impact on plant growth and development remains unexplored. Creeping bentgrass (Agrostis stolonifera L.) is a C3 cool‐season perennial
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Multiplexed silencing of 2S albumin genes in peanut Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-08 Joann A. Conner, Larissa Arrais Guimaraes, Zhifen Zhang, Kathleen Marasigan, Ye Chu, Walid Korani, Peggy Ozias‐Akins
Peanut (Arachis hypogaea L.) is a highly nutritious legume that provides energy-dense food. One obstacle for universal inclusion of peanut products in the diet is the prevalence of peanut food allergy. Although approximately 2% of individuals may become sensitized to peanut and experience an allergic reaction upon ingestion (Gupta et al., 2011), a minor fraction of these reactions may be life-threatening
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Insights into spinach domestication from genome sequences of two wild spinach progenitors, Spinacia turkestanica and Spinacia tetrandra New Phytol. (IF 9.4) Pub Date : 2024-05-08 Hongbing She, Zhiyuan Liu, Zhaosheng Xu, Helong Zhang, Jian Wu, Xiaowu Wang, Feng Cheng, Deborah Charlesworth, Wei Qian
Summary Cultivated spinach (Spinacia oleracea) is a dioecious species. We report high‐quality genome sequences for its two closest wild relatives, Spinacia turkestanica and Spinacia tetrandra, which are also dioecious, and are used to study the genetics of spinach domestication. Using a combination of genomic approaches, we assembled genomes of both these species and analyzed them in comparison with
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Nectar and floral morphology differ in evolutionary potential in novel pollination environments New Phytol. (IF 9.4) Pub Date : 2024-05-08 Andrés Romero‐Bravo, Maria Clara Castellanos
Summary Plants can evolve rapidly after pollinator changes, but the response of different floral traits to novel selection can vary. Floral morphology is often expected to show high integration to maintain pollination accuracy, while nectar traits can be more environmentally sensitive. The relative role of genetic correlations and phenotypic plasticity (PP) in floral evolution remains unclear, particularly
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CRWN nuclear lamina components maintain the H3K27me3 landscape and promote successful reproduction in Arabidopsis New Phytol. (IF 9.4) Pub Date : 2024-05-08 Junsik Choi, Mary Gehring
Summary Arabidopsis lamin analogs CROWDED NUCLEIs (CRWNs) are necessary to maintain nuclear structure, genome function, and proper plant growth. However, whether and how CRWNs impact reproduction and genome‐wide epigenetic modifications is unknown. Here, we investigate the role of CRWNs during the development of gametophytes, seeds, and endosperm, using genomic and epigenomic profiling methods. We
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PSEUDO-RESPONSE REGULATOR 3b and transcription factor ABF3 modulate abscisic acid-dependent drought stress response in soybean Plant Physiol. (IF 7.4) Pub Date : 2024-05-08 Cong Li, Yanhang Chen, Qing Hu, Xiaolan Yang, Yunfeng Zhao, Yan Lin, Jianbo Yuan, Jinbao Gu, Yang Li, Jin He, Dong Wang, Bin Liu, Zhen-Yu Wang
The circadian system plays a pivotal role in facilitating the ability of crop plants to respond and adapt to fluctuations in their immediate environment effectively. Despite the increasing comprehension of PSEUDO-RESPONSE REGULATORs (PRRs) and their involvement in the regulation of diverse biological processes, including circadian rhythms, photoperiodic control of flowering, and responses to abiotic
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Liquid–liquid phase separation of TZP promotes PPK-mediated phosphorylation of the phytochrome A photoreceptor Nat. Plants (IF 18.0) Pub Date : 2024-05-07 Ziyi Feng, Meijiao Wang, Yan Liu, Cong Li, Shaoman Zhang, Jie Duan, Jiaqi Chen, Lijuan Qi, Yanru Liu, Hong Li, Jie Wu, Yannan Liu, William Terzaghi, Feng Tian, Bojian Zhong, Xiaofeng Fang, Weiqiang Qian, Yan Guo, Xing Wang Deng, Jigang Li
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Expanding plant genome editing scope and profiles with CRISPR‐FrCas9 systems targeting palindromic TA sites Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-07 Yao He, Yangshuo Han, Yanqin Ma, Shishi Liu, Tingting Fan, Yanling Liang, Xu Tang, Xuelian Zheng, Yuechao Wu, Tao Zhang, Yiping Qi, Yong Zhang
SummaryCRISPR‐Cas9 is widely used for genome editing, but its PAM sequence requirements limit its efficiency. In this study, we explore Faecalibaculum rodentium Cas9 (FrCas9) for plant genome editing, especially in rice. FrCas9 recognizes a concise 5′‐NNTA‐3′ PAM, targeting more abundant palindromic TA sites in plant genomes than the 5′‐NGG‐3′ PAM sites of the most popular SpCas9. FrCas9 shows cleavage
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A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901 Plant Physiol. (IF 7.4) Pub Date : 2024-05-07 Angelo J Victoria, Tiago Toscano Selão, José Ángel Moreno-Cabezuelo, Lauren A Mills, Grant A R Gale, David J Lea-Smith, Alistair J McCormick
Synechococcus sp. PCC 11901 (PCC 11901) is a fast-growing marine cyanobacterial strain that has a capacity for sustained biomass accumulation to very high cell densities, comparable to that achieved by commercially relevant heterotrophic organisms. However, genetic tools to engineer PCC 11901 for biotechnology applications are limited. Here we describe a suite of tools based on the CyanoGate MoClo
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Broad range plastid genome editing with monomeric TALE‐linked cytosine and dual base editors Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-06 Xiaoyu Wang, Tyson Fang, Jason Lu, Leena Tripathi, Yiping Qi
Editing of the plastid genome helps understand the molecular functions of plastid genes and engineer desired traits in crops (Maliga, 2022). The DddA-derived cytosine base editors (DdCBEs) enable C-to-T editing in mitochondrial and plastid genomes (Kang et al., 2021; Li et al., 2021; Mok et al., 2020; Nakazato et al., 2021). Recently, Cho et al. (2022) developed TALE-linked deaminases (TALED) that
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Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803 New Phytol. (IF 9.4) Pub Date : 2024-05-06 Pablo Ortega‐Martínez, Lauri Nikkanen, Laura T. Wey, Francisco J. Florencio, Yagut Allahverdiyeva, Sandra Díaz‐Troya
Summary Some cyanobacteria can grow photoautotrophically or photomixotrophically by using simultaneously CO2 and glucose. The switch between these trophic modes and the role of glycogen, their main carbon storage macromolecule, was investigated. We analysed the effect of glucose addition on the physiology, metabolic and photosynthetic state of Synechocystis sp. PCC 6803 and mutants lacking phosphoglucomutase
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The varied forms and functions of plasmodesmata New Phytol. (IF 9.4) Pub Date : 2024-05-06 Tessa M. Burch‐Smith
Plasmodesmata provide routes for the movement of various molecules between plant cells. Cargo molecules of plasmodesmata include metabolites like sucrose, small signaling molecules like hormones, and large endogenous signaling molecules like mRNAs. This diversity in cargo lends support to the view that plasmodesmata are essential for plant growth, development, and responses to the environment. Due
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Unveiling the transferability of PLSR models for leaf trait estimation: lessons from a comprehensive analysis with a novel global dataset New Phytol. (IF 9.4) Pub Date : 2024-05-06 Fujiang Ji, Fa Li, Dalei Hao, Alexey N. Shiklomanov, Xi Yang, Philip A. Townsend, Hamid Dashti, Tatsuro Nakaji, Kyle R. Kovach, Haoran Liu, Meng Luo, Min Chen
Summary Leaf traits are essential for understanding many physiological and ecological processes. Partial least squares regression (PLSR) models with leaf spectroscopy are widely applied for trait estimation, but their transferability across space, time, and plant functional types (PFTs) remains unclear. We compiled a novel dataset of paired leaf traits and spectra, with 47 393 records for > 700 species
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A bamboo ‘PeSAPK4‐PeMYB99‐PeTIP4‐3’ regulatory model involved in water transport New Phytol. (IF 9.4) Pub Date : 2024-05-06 Chenglei Zhu, Zeming Lin, Kebin Yang, Yongfeng Lou, Yan Liu, Tiankuo Li, Hui Li, Xiaolin Di, Jiangfei Wang, Huayu Sun, Ying Li, Xueping Li, Zhimin Gao
Summary Water plays crucial roles in expeditious growth and osmotic stress of bamboo. Nevertheless, the molecular mechanism of water transport remains unclear. In this study, an aquaporin gene, PeTIP4‐3, was identified through a joint analysis of root pressure and transcriptomic data in moso bamboo (Phyllostachys edulis). PeTIP4‐3 was highly expressed in shoots, especially in the vascular bundle sheath